論文 - 詳細
| RRC ID | 38723 |
|---|---|
| 著者 | Kashii T, Mizushima Y, Monno S, Nakagawa K, Kobayashi M. |
| タイトル | Gene analysis of K-, H-ras, p53, and retinoblastoma susceptibility genes in human lung cancer cell lines by the polymerase chain reaction/single-strand conformation polymorphism method. |
| ジャーナル | J Cancer Res Clin Oncol |
| Abstract |
In order to know the involvement of multiple gene alterations in the pathogenesis of human lung cancer, we examined the genes of K-, H-ras (codons 12, 13, 61), p53(exons 5-9) and the retinoblastoma susceptibility gene (RB)(exons 20-22) using the polymerase chain reaction/single-strand conformation polymorphism method in 32 human lung cancer cell lines (5 squamous-cell carcinomas, 10 adenocarcinomas, 3 large-cell carcinomas, 14 small-cell carcinomas). In 18 non-small-cell lung cancer lines, gene alterations were found in 4 for K-ras (22%), none for H-ras (0%), 4 for p53 (22%) and none for the RB (0%) gene. In 14 small-cell lung cancer (SCLC) lines, no gene alterations were found in K-ras (0%), or H-ras (0%), but 6 were found for p53 (43%) and 3 for the RB (21%) gene. Coincident abnormalities of K-ras and p53, or K-ras and RB genes were not found in any cell lines, and those of the p53 and RB genes were found in only 2 SCLC lines. No association was observed between these three gene alterations and N-myc amplification. Although the above three genes may be involved to some extent in the pathogenesis of lung cancer, more factors are required for its development. |
| 巻・号 | 120(3) |
| ページ | 143-8 |
| 公開日 | 1994-1-1 |
| DOI | 10.1007/BF01202192 |
| PMID | 8263009 |
| MeSH | Aged Base Sequence Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Small Cell / genetics* Female Genes, Retinoblastoma / genetics* Genes, myc / genetics Genes, p53 / genetics* Genes, ras / genetics* Humans Lung Neoplasms / genetics* Male Middle Aged Molecular Sequence Data Point Mutation / genetics* Polymerase Chain Reaction / methods Sequence Analysis, DNA Tumor Cells, Cultured |
| IF | 3.656 |
| 引用数 | 36 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | human lung cancer cell |