RRC ID 38723
Author Kashii T, Mizushima Y, Monno S, Nakagawa K, Kobayashi M.
Title Gene analysis of K-, H-ras, p53, and retinoblastoma susceptibility genes in human lung cancer cell lines by the polymerase chain reaction/single-strand conformation polymorphism method.
Journal J. Cancer Res. Clin. Oncol.
Abstract In order to know the involvement of multiple gene alterations in the pathogenesis of human lung cancer, we examined the genes of K-, H-ras (codons 12, 13, 61), p53(exons 5-9) and the retinoblastoma susceptibility gene (RB)(exons 20-22) using the polymerase chain reaction/single-strand conformation polymorphism method in 32 human lung cancer cell lines (5 squamous-cell carcinomas, 10 adenocarcinomas, 3 large-cell carcinomas, 14 small-cell carcinomas). In 18 non-small-cell lung cancer lines, gene alterations were found in 4 for K-ras (22%), none for H-ras (0%), 4 for p53 (22%) and none for the RB (0%) gene. In 14 small-cell lung cancer (SCLC) lines, no gene alterations were found in K-ras (0%), or H-ras (0%), but 6 were found for p53 (43%) and 3 for the RB (21%) gene. Coincident abnormalities of K-ras and p53, or K-ras and RB genes were not found in any cell lines, and those of the p53 and RB genes were found in only 2 SCLC lines. No association was observed between these three gene alterations and N-myc amplification. Although the above three genes may be involved to some extent in the pathogenesis of lung cancer, more factors are required for its development.
Volume 120(3)
Pages 143-8
Published 1994
DOI 10.1007/bf01202192
PMID 8263009
MeSH Aged Base Sequence Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Small Cell / genetics* Female Genes, Retinoblastoma / genetics* Genes, myc / genetics Genes, p53 / genetics* Genes, ras / genetics* Humans Lung Neoplasms / genetics* Male Middle Aged Molecular Sequence Data Point Mutation / genetics* Polymerase Chain Reaction / methods Sequence Analysis, DNA Tumor Cells, Cultured
IF 3.282
Times Cited 34
Human and Animal Cells