論文 - 詳細
| RRC ID | 38726 |
|---|---|
| 著者 | Yoshihara K, Tsuyuki M, Itaya A, Tanaka Y, Kamiya T. |
| タイトル | 3-aminobenzamide, a potent inhibitor of poly (ADP-ribose) polymerase, causes a rapid death of HL-60 cells cultured in serum-free medium. |
| ジャーナル | Mol Cell Biochem |
| Abstract |
HL-60 cells transferred from serum-supplemented to serum-free culture medium initially bound to culture plate tightly and then released from the plate on increasing the culture time and resumed exponential growth after about 8 h lag. At the initial stage of the culture, the cells became extremely sensitive to 3-aminobenzamide, a potent inhibitor of poly (ADP-ribose) polymerase, and, at 1 mM, 80 to 90% of the cells were lysed within 20 h, whereas the inhibitor was totally ineffective on the cell growth in serum-supplemented medium at the concentration. Non-inhibitory analogs of the inhibitor were ineffective. Assay of poly(ADP-ribose) polymerase activity in permeable cells indicated that a transient activation of the enzyme occurred during the culture in serum-free medium (the maximum activation was observed at 8 h of the culture). The cells conditioned in serum-free medium for 24 h acquired significant resistancy to the inhibitor. A low concentration of fibronectin (5 to 10 micrograms/ml) and a relatively high concentration of bovine serum albumin (0.5 to 1 mg/ml) effectively blocked the cell attachment to plate and also the 3-aminobenzamide-induced cell lysis. These results suggest that poly(ADP-ribose) polymerase is involved in a process essential for HL-60 cells to adapt to a serum-deprived growth condition. |
| 巻・号 | 135(2) |
| ページ | 143-51 |
| 公開日 | 1994-6-29 |
| DOI | 10.1007/BF00926517 |
| PMID | 7838142 |
| MeSH | Benzamides / pharmacology* Cell Adhesion / drug effects Cell Death / drug effects Cell Division / drug effects Culture Media, Serum-Free / pharmacology* Fibronectins / pharmacology Leukemia, Promyelocytic, Acute / pathology* Neoplasm Proteins / antagonists & inhibitors* Poly(ADP-ribose) Polymerase Inhibitors* Serum Albumin, Bovine / pharmacology Time Factors Tumor Cells, Cultured / drug effects |
| IF | 2.795 |
| 引用数 | 3 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |