| RRC ID |
38726
|
| 著者 |
Yoshihara K, Tsuyuki M, Itaya A, Tanaka Y, Kamiya T.
|
| タイトル |
3-aminobenzamide, a potent inhibitor of poly (ADP-ribose) polymerase, causes a rapid death of HL-60 cells cultured in serum-free medium.
|
| ジャーナル |
Mol Cell Biochem
|
| Abstract |
HL-60 cells transferred from serum-supplemented to serum-free culture medium initially bound to culture plate tightly and then released from the plate on increasing the culture time and resumed exponential growth after about 8 h lag. At the initial stage of the culture, the cells became extremely sensitive to 3-aminobenzamide, a potent inhibitor of poly (ADP-ribose) polymerase, and, at 1 mM, 80 to 90% of the cells were lysed within 20 h, whereas the inhibitor was totally ineffective on the cell growth in serum-supplemented medium at the concentration. Non-inhibitory analogs of the inhibitor were ineffective. Assay of poly(ADP-ribose) polymerase activity in permeable cells indicated that a transient activation of the enzyme occurred during the culture in serum-free medium (the maximum activation was observed at 8 h of the culture). The cells conditioned in serum-free medium for 24 h acquired significant resistancy to the inhibitor. A low concentration of fibronectin (5 to 10 micrograms/ml) and a relatively high concentration of bovine serum albumin (0.5 to 1 mg/ml) effectively blocked the cell attachment to plate and also the 3-aminobenzamide-induced cell lysis. These results suggest that poly(ADP-ribose) polymerase is involved in a process essential for HL-60 cells to adapt to a serum-deprived growth condition.
|
| 巻・号 |
135(2)
|
| ページ |
143-51
|
| 公開日 |
1994-6-29
|
| DOI |
10.1007/BF00926517
|
| PMID |
7838142
|
| MeSH |
Benzamides / pharmacology*
Cell Adhesion / drug effects
Cell Death / drug effects
Cell Division / drug effects
Culture Media, Serum-Free / pharmacology*
Fibronectins / pharmacology
Leukemia, Promyelocytic, Acute / pathology*
Neoplasm Proteins / antagonists & inhibitors*
Poly(ADP-ribose) Polymerase Inhibitors*
Serum Albumin, Bovine / pharmacology
Time Factors
Tumor Cells, Cultured / drug effects
|
| IF |
2.795
|
| 引用数 |
3
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
