論文 - 詳細
| RRC ID | 38737 |
|---|---|
| 著者 | Kurimoto M, Endo S, Arai K, Horie Y, Nogami K, Takaku A. |
| タイトル | TM-1 cells from an established human malignant glioma cell line produce PDGF, TGF-alpha, and TGF-beta which cooperatively play a stimulatory role for an autocrine growth promotion. |
| ジャーナル | J Neurooncol |
| Abstract |
We have previously established a human malignant glioma cell line, TM-1. TM-1 cells could proliferate in the serum-free medium. In the present study, immunochemical analysis demonstrated that platelet-derived growth factor (PDGF), transforming growth factor (TGF)-alpha, and TGF-beta are present in the serum-free medium conditioned by growing TM-1 cells. While the cells appeared to possess a single type of binding sites for epidermal growth factor (EGF) with properties comparable to those determined for other tumor cells, the conditioned medium did not contain EGF.PDGF, TGF-alpha, and EGF added exogenously to serum-free media stimulated thymidine incorporation into DNA of TM-1 cells. In addition, antibodies specific for PDGF and TGF-alpha suppressed this activity. These results indicate autocrine and stimulatory roles of PDGF and TGF-alpha for the proliferation of TM-1 cells. As observed for other tumor cells, TGF-beta by itself weakly suppressed thymidine incorporation by TM-1 cells. However, TGF-beta employed in combination with TGF-alpha or EGF appeared to stimulate thymidine incorporation, suggesting that a cooperative action of TGF-beta with different growth factors may be involved in the stimulatory growth regulation at least for TM-1 cells. |
| 巻・号 | 22(1) |
| ページ | 33-44 |
| 公開日 | 1994-1-1 |
| DOI | 10.1007/BF01058353 |
| PMID | 7714549 |
| MeSH | Cell Division DNA / biosynthesis ErbB Receptors / metabolism Glioma / metabolism* Glioma / pathology Humans Platelet-Derived Growth Factor / metabolism* Transforming Growth Factor alpha / metabolism* Transforming Growth Factor beta / metabolism* Tumor Cells, Cultured |
| IF | 3.267 |
| 引用数 | 11 |
| WOS 分野 | ONCOLOGY CLINICAL NEUROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | NRK49F(RCB0112) |