RRC ID 38737
著者 Kurimoto M, Endo S, Arai K, Horie Y, Nogami K, Takaku A.
タイトル TM-1 cells from an established human malignant glioma cell line produce PDGF, TGF-alpha, and TGF-beta which cooperatively play a stimulatory role for an autocrine growth promotion.
ジャーナル J Neurooncol
Abstract We have previously established a human malignant glioma cell line, TM-1. TM-1 cells could proliferate in the serum-free medium. In the present study, immunochemical analysis demonstrated that platelet-derived growth factor (PDGF), transforming growth factor (TGF)-alpha, and TGF-beta are present in the serum-free medium conditioned by growing TM-1 cells. While the cells appeared to possess a single type of binding sites for epidermal growth factor (EGF) with properties comparable to those determined for other tumor cells, the conditioned medium did not contain EGF.PDGF, TGF-alpha, and EGF added exogenously to serum-free media stimulated thymidine incorporation into DNA of TM-1 cells. In addition, antibodies specific for PDGF and TGF-alpha suppressed this activity. These results indicate autocrine and stimulatory roles of PDGF and TGF-alpha for the proliferation of TM-1 cells. As observed for other tumor cells, TGF-beta by itself weakly suppressed thymidine incorporation by TM-1 cells. However, TGF-beta employed in combination with TGF-alpha or EGF appeared to stimulate thymidine incorporation, suggesting that a cooperative action of TGF-beta with different growth factors may be involved in the stimulatory growth regulation at least for TM-1 cells.
巻・号 22(1)
ページ 33-44
公開日 1994-1-1
DOI 10.1007/BF01058353
PMID 7714549
MeSH Cell Division DNA / biosynthesis ErbB Receptors / metabolism Glioma / metabolism* Glioma / pathology Humans Platelet-Derived Growth Factor / metabolism* Transforming Growth Factor alpha / metabolism* Transforming Growth Factor beta / metabolism* Tumor Cells, Cultured
IF 3.267
引用数 11
WOS 分野 ONCOLOGY CLINICAL NEUROLOGY
リソース情報
ヒト・動物細胞 NRK49F(RCB0112)