論文 - 詳細
| RRC ID | 38738 |
|---|---|
| 著者 | Takeda T, Nakajima K, Kojima H, Hirano T. |
| タイトル | E1A repression of IL-6-induced gene activation by blocking the assembly of IL-6 response element binding complexes. |
| ジャーナル | J Immunol |
| Abstract |
Some viral products interfere with host antiviral defense mechanisms. Adenovirus E1A represses IFN signal transduction pathways which induces gene activation and an antiviral state. Both IFN and IL-6 activate Jak/Tyk protein tyrosine kinases and the STAT (signal transducer and activator of transcription) family proteins. We showed that 12S E1A repressed IL-6 signals activating the junB promoter and the two IL-6 response elements (REs), JRE-IL6 and type II IL-6 RE (also called acute phase response element), required for IL-6-induced activation of the junB promoter and the type II acute phase reactant genes, respectively, in hepatocytes. Conserved region 1 of the 12S E1A was responsible for the repression. Target molecules of the repression by E1A appeared to be IL-6-inducible DNA-binding proteins acting on the IL-6 REs. In a rat 3Y1 cell line stably expressing E1A, the levels of IL-6-induced IL-6 RE binding proteins were severely reduced compared with those in a parental 3Y1 cell line. Moreover, we found that the levels of the STAT family proteins including Stat1-alpha (p91), Stat1-beta (p84), Stat2 (p113), and Stat3 were decreased by the stable expression of adenovirus E1A. The E1A-induced reduction in the amount of DNA-binding proteins seemed to be partly responsible for the decreased transcriptional activity of the IL-6 RE-driven gene expression in response to IL-6. This repression mechanism may be applicable to the E1A repression of IFN-gamma-induced gene activation. |
| 巻・号 | 153(10) |
| ページ | 4573-82 |
| 公開日 | 1994-11-15 |
| PMID | 7963529 |
| MeSH | Adenovirus E1A Proteins / physiology* Animals Base Sequence Blotting, Northern Blotting, Western Cell Line DNA-Binding Proteins / metabolism* Down-Regulation / physiology* Electrophoresis, Polyacrylamide Gel / methods Genes, jun / genetics Interleukin-6 / metabolism* Molecular Sequence Data Promoter Regions, Genetic Rats Regulatory Sequences, Nucleic Acid* Transfection |
| IF | 4.886 |
| 引用数 | 32 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 3Y1-B clone 1-6(RCB0488) E1A-3Y1-1(RCB0332) |