RRC ID 38747
Author Adachi Y, Okazaki M, Ohno N, Yadomae T.
Title Enhancement of cytokine production by macrophages stimulated with (1-->3)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa.
Journal Biol Pharm Bull
Abstract The ability of grifolan (GRN), a purified fungal (1-->3)-beta-D-glucan, to induce various cytokines from macrophages was examined in vitro. Interleukin-6 (IL-6) activity in supernatants from the culture of macrophage cell line, RAW264.7 was dependent on increasing doses of GRN. The level of IL-6 induced with 500 micrograms/ml of GRN was comparable to that induced with lipopolysaccharide (LPS) 10 micrograms/ml. Enhancement of the mRNA level of IL-6 by treatment with GRN was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The effect of GRN on production of IL-6 was also observed using peritoneal macrophages from C3H/HeJ mice which did not respond to endotoxins. This data suggested that the ability of GRN to activate IL-6 production of macrophages is not due to contamination of endotoxins in the preparation. Enhanced production of cytokine by GRN was observed not only with IL-6, but also with interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha). In the production of TNF alpha, GRN was more effective than LPS used in this study. Other soluble or gel-forming(1-->3)-beta-D-glucans from various sources did not enhance the production of such cytokines although they are structurally similar to GRN. The above results indicate that GRN is a novel macrophage activator which augments cytokine production without dependence on endotoxins.
Volume 17(12)
Pages 1554-60
Published 1994-12-1
DOI 10.1248/bpb.17.1554
PMID 7537572
MeSH Adjuvants, Immunologic / isolation & purification Adjuvants, Immunologic / pharmacology* Animals Base Sequence Cytokines / biosynthesis* Glucans / isolation & purification Glucans / pharmacology In Vitro Techniques Interleukin-1 / biosynthesis Interleukin-6 / biosynthesis Lipopolysaccharides / pharmacology Macrophages, Peritoneal / drug effects Macrophages, Peritoneal / metabolism* Mice Mice, Inbred C3H Molecular Sequence Data Plants, Medicinal / chemistry* Polymerase Chain Reaction RNA-Directed DNA Polymerase T-Lymphocytes / drug effects Tumor Necrosis Factor-alpha / biosynthesis beta-Glucans*
IF 1.863
Times Cited 159
WOS Category PHARMACOLOGY & PHARMACY
Resource
Human and Animal Cells RAW 264(RCB0535)