論文 - 詳細
| RRC ID | 38748 |
|---|---|
| 著者 | Suzu S, Kimura F, Yamada M, Yanai N, Kawashima T, Nagata N, Motoyoshi K. |
| タイトル | Direct interaction of proteoglycan macrophage colony-stimulating factor and basic fibroblast growth factor. |
| ジャーナル | Blood |
| Abstract |
The proteoglycan form of macrophage colony-stimulating factor (PG-M-CSF), but not M-CSF with a molecular weight of 85 kD (85-kD M-CSF), bound to immobilized basic fibroblast growth factor (bFGF), and, conversely, bFGF bound to immobilized PG-M-CSF, but not to the 85-kD M-CSF. PG-M-CSF has an additional amino acid sequence at its carboxyl terminus (part of a precursor sequence that is removed in 85-kD M-CSF by proteolytic processing) and it has one or two chondroitin sulfate glycosaminoglycan chains at the carboxyl terminus. Enzymatic removal of the chondroitin sulfate chain from PG-M-CSF had no effect on the binding between PG-M-CSF and bFGF. Ligand blotting analysis with radioiodinated bFGF showed that bFGF specifically bound to the polypeptide that corresponded to the carboxyl terminus of PG-M-CSF and was produced in Escherichia coli transfected with its gene. The exogeneous addition of heparan sulfate, which has strong affinity for bFGF, efficiently inhibited the binding between PG-M-CSF and bFGF. These results show that PG-M-CSF binds bFGF through its carboxyl terminal peptide and that the binding sites for PG-M-CSF and heparan sulfate on bFGF are located close together. PG-M-CSF also significantly reduced the mitogenic action of bFGF on Balb/c 3T3 mouse fibroblastic cells. Therefore, we conclude that PG-M-CSF not only binds bFGF, but also neutralizes the activity of the growth factor. |
| 巻・号 | 83(11) |
| ページ | 3113-9 |
| 公開日 | 1994-6-1 |
| PII | S0006-4971(20)78891-9 |
| PMID | 8193348 |
| MeSH | 3T3 Cells Animals Chondroitin Sulfate Proteoglycans / metabolism* Chondroitin Sulfate Proteoglycans / pharmacology DNA / biosynthesis Fibroblast Growth Factor 2 / antagonists & inhibitors Fibroblast Growth Factor 2 / metabolism* Macrophage Colony-Stimulating Factor / metabolism* Macrophage Colony-Stimulating Factor / pharmacology Mice |
| IF | 17.794 |
| 引用数 | 14 |
| WOS 分野 | HEMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |