| RRC ID |
38750
|
| Author |
Nishihara T, Akifusa S, Koseki T, Kato S, Muro M, Hanada N.
|
| Title |
Specific inhibitors of vacuolar type H(+)-ATPases induce apoptotic cell death.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Concanamycin A and bafilomycin A1 are known as strong inhibitors of the vacuolar type H(+)-ATPases in vitro. These inhibitors exhibited cytotoxic effects on twelve cell lines in cell viability assay. On the other hand, the F1F0-type H(+)-ATPase inhibitor oligomycin and the E1E2-type H(+)-ATPase inhibitor vanadate showed no cytotoxic effect. We show here that concanamycin A and bafilomycin A1 induce a significant increase in the proportion of fragmented DNA in agarose gel electrophoresis. Flow cytometric cell cycle analysis of WEHI 231 cells stimulated with concanamycin A revealed the increased percentage of apoptotic cells with hypodiploid DNA. These findings indicate that cell death induced by specific inhibitors of vacuolar type H(+)-ATPases occurs through apoptosis.
|
| Volume |
212(1)
|
| Pages |
255-62
|
| Published |
1995-7-6
|
| DOI |
10.1006/bbrc.1995.1964
|
| PII |
S0006-291X(85)71964-X
|
| PMID |
7612014
|
| MeSH |
Animals
Anti-Bacterial Agents / pharmacology*
Apoptosis / drug effects*
Cell Line
Cell Survival / drug effects
DNA, Neoplasm / drug effects
DNA, Neoplasm / isolation & purification
Dose-Response Relationship, Drug
Flow Cytometry
Humans
Macrolides*
Mice
Proton-Translocating ATPases / antagonists & inhibitors*
Tumor Cells, Cultured
Vacuoles / enzymology*
|
| IF |
2.985
|
| Times Cited |
63
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB0459) |
