Reference - Detail
| RRC ID | 38750 |
|---|---|
| Author | Nishihara T, Akifusa S, Koseki T, Kato S, Muro M, Hanada N. |
| Title | Specific inhibitors of vacuolar type H(+)-ATPases induce apoptotic cell death. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Concanamycin A and bafilomycin A1 are known as strong inhibitors of the vacuolar type H(+)-ATPases in vitro. These inhibitors exhibited cytotoxic effects on twelve cell lines in cell viability assay. On the other hand, the F1F0-type H(+)-ATPase inhibitor oligomycin and the E1E2-type H(+)-ATPase inhibitor vanadate showed no cytotoxic effect. We show here that concanamycin A and bafilomycin A1 induce a significant increase in the proportion of fragmented DNA in agarose gel electrophoresis. Flow cytometric cell cycle analysis of WEHI 231 cells stimulated with concanamycin A revealed the increased percentage of apoptotic cells with hypodiploid DNA. These findings indicate that cell death induced by specific inhibitors of vacuolar type H(+)-ATPases occurs through apoptosis. |
| Volume | 212(1) |
| Pages | 255-62 |
| Published | 1995-7-6 |
| DOI | 10.1006/bbrc.1995.1964 |
| PII | S0006-291X(85)71964-X |
| PMID | 7612014 |
| MeSH | Animals Anti-Bacterial Agents / pharmacology* Apoptosis / drug effects* Cell Line Cell Survival / drug effects DNA, Neoplasm / drug effects DNA, Neoplasm / isolation & purification Dose-Response Relationship, Drug Flow Cytometry Humans Macrolides* Mice Proton-Translocating ATPases / antagonists & inhibitors* Tumor Cells, Cultured Vacuoles / enzymology* |
| IF | 2.985 |
| Times Cited | 63 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2(RCB0459) |