論文 - 詳細
| RRC ID | 38756 |
|---|---|
| 著者 | Kanzaki M, Shibata H, Mogami H, Kojima I. |
| タイトル | Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells. |
| ジャーナル | J Biol Chem |
| Abstract |
CD20 is a transmembrane protein that functions as a Ca(2+)-permeable cation channel (Bubien, J. K., Zhou, L. J., Bell, P. D., Frizzel, R. A., and Tedder, T. F. (1993) J. Cell Biol. 121, 1121-1132) and is involved in growth regulation of B lymphocytes. In order to further investigate the role of calcium entry in cell cycle progression, we introduced the cDNA encoding a Ca(2+)-permeable cation channel, CD20, into Balb/c 3T3 cells. Balb/c 3T3 cells transfected with a vector containing cDNA encoding CD20 expressed the CD20 protein, which was detected by assaying the binding of a monoclonal antibody against CD20. Calcium-permeable cation channel activity was detected in CD20-expressing cells by whole cell patch clamp recording and microfluorometric determination of the cytoplasmic Ca2+ concentration using fura-2. The expression of CD20 induced significant alterations in the responses of the cells to insulin-like growth factor-I (IGF-I). IGF-I induced DNA synthesis by control cells only when they had been pretreated with both platelet-derived growth factor (PDGF) and epidermal growth factor (EGF). In contrast, DNA synthesis by 30% of the quiescent CD20-expressing cells was initiated in response to IGF-I in the absence of priming with PDGF and EGF. When control quiescent cells were primed with PDGF and EGF, the addition of IGF-I led to the initiation of DNA synthesis after 14 h or more, whereas it induced DNA synthesis by CD20-expressing cells primed with PDGF and EGF 4 h earlier. The IGF-induced DNA synthesis was dependent on extracellular Ca2+, and expression of CD20 reduced the concentration of extracellular Ca2+ required for it. Furthermore, DNA synthesis by approximately 25% of the CD20-expressing cells was initiated after priming with PDGF and EGF, even in the absence of the progression factor IGF-I. These results indicate that CD20 expressed in Balb/c 3T3 cells functions as a constitutively active Ca(2+)-permeable cation channel and that expression of CD20 accelerates G1 progression in a Ca(2+)-dependent manner. |
| 巻・号 | 270(22) |
| ページ | 13099-104 |
| 公開日 | 1995-6-2 |
| DOI | 10.1074/jbc.270.22.13099 |
| PII | S0021-9258(18)92251-9 |
| PMID | 7539422 |
| MeSH | 3T3 Cells Animals Antigens, CD / genetics Antigens, CD / physiology* Antigens, CD20 Antigens, Differentiation, B-Lymphocyte / genetics Antigens, Differentiation, B-Lymphocyte / physiology* Base Sequence Calcium / physiology* Calcium Channels / genetics Calcium Channels / physiology* Cations DNA Primers DNA Replication / genetics DNA, Complementary G1 Phase / genetics G1 Phase / physiology* Insulin-Like Growth Factor I / physiology Mice Mice, Inbred BALB C Molecular Sequence Data Phosphorylation Transfection |
| IF | 4.238 |
| 引用数 | 65 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |