論文 - 詳細
RRC ID | 38766 |
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著者 | Numazawa S, Honma Y, Yamamoto T, Yoshida T, Kuroiwa Y. |
タイトル | A cardiotonic steroid bufalin-like factor in human plasma induces leukemia cell differentiation. |
ジャーナル | Leuk Res |
Abstract |
A Na+,K(+)-ATPase inhibitor, bufalin, has been shown previously to induce leukemia cell differentiation. The presence of a circulating Na+,K(+)-ATPase inhibitor has been proposed in mammals. The aim of this study was to explore an endogenous bufalin-like factor that induces leukemia cell differentiation. We found a fraction, designated as fraction A, obtained from human plasma extract that inhibits the growth of several human-derived leukemia cell lines. The effect of the fraction was retained after protease digestion or heat treatment. Murine leukemia cells and ouabain-resistant cells, which are insensitive to bufalin, appeared to be refractory to fraction A in terms of growth inhibition. Fraction A also induced functional and morphological maturation in THP-1 cells. Fraction A was recognized by anti-bufalin anti-serum and inhibited 3H-bufalin binding to K562 cells. These findings suggest that fraction A shows a similar behavior to that of bufalin on leukemia cells by inhibiting Na+,K(+)-ATPase. We propose that an endogenous Na+,K(+)-ATPase inhibitor in human plasma may play a role in cell differentiation. |
巻・号 | 19(12) |
ページ | 945-53 |
公開日 | 1995-12-1 |
DOI | 10.1016/0145-2126(95)00081-x |
PII | 014521269500081X |
PMID | 8632664 |
MeSH | Antineoplastic Agents / blood Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Biological Factors / blood Biological Factors / isolation & purification Biological Factors / pharmacology* Bufanolides / blood Bufanolides / isolation & purification Bufanolides / pharmacology* Cardiotonic Agents / blood Cardiotonic Agents / isolation & purification Cardiotonic Agents / pharmacology* Cell Differentiation / drug effects Chemical Fractionation Chromatography, High Pressure Liquid Enzyme Inhibitors / blood Enzyme Inhibitors / isolation & purification Enzyme Inhibitors / pharmacology Humans Leukemia / pathology* Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / pathology |
IF | 2.214 |
引用数 | 22 |
WOS 分野 | ONCOLOGY HEMATOLOGY |
リソース情報 | |
ヒト・動物細胞 | HL60(RCB0041) |