| RRC ID |
38780
|
| 著者 |
Sasaki H, Nagura K, Ishino M, Tobioka H, Kotani K, Sasaki T.
|
| タイトル |
Cloning and characterization of cell adhesion kinase beta, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
A second protein-tyrosine kinase (PTK) of the focal adhesion kinase (FAK) subfamily, cell adhesion kinase beta (CAK beta), was identified by cDNA cloning. The rat CAK beta is a 115.7-kDa PTK that contains N- and C-terminal domains of 418 and 330 amino acid residues besides the central kinase domain. The rat CAK beta has a homology with mouse FAK over their entire lengths except for the extreme N-terminal 88 residues and shares 45% overall sequence identity (60% identical in the catalytic domain), which indicates that CAK beta is a protein structurally related to but different from FAK. The CAK beta gene is less evenly expressed in a variety of rat organs than the FAK gene. Anti-CAK beta antibody immunoprecipitated a 113-kDa protein from rat brain, 3Y1 fibroblasts, and COS-7 cells transfected with CAK beta cDNA. The tyrosine-phosphorylated state of CAK beta was not reduced on trypsinization, nor enhanced in response to plating 3Y1 cells onto fibronectin. CAK beta localized to sites of cell-to-cell contact in COS-7 transfected with CAK beta cDNA, in which FAK was found at the bottom of the cells. Thus, CAK beta is a PTK possibly participating in the signal transduction regulated by cell-to-cell contacts.
|
| 巻・号 |
270(36)
|
| ページ |
21206-19
|
| 公開日 |
1995-9-8
|
| DOI |
10.1074/jbc.270.36.21206
|
| PII |
S0021-9258(18)90339-X
|
| PMID |
7673154
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Brain / enzymology
Cell Adhesion Molecules / genetics*
Cell Adhesion Molecules / metabolism
Cell Line
Cloning, Molecular
DNA, Complementary
Focal Adhesion Kinase 1
Focal Adhesion Kinase 2
Focal Adhesion Protein-Tyrosine Kinases
Humans
Molecular Sequence Data
Phosphorylation
Protein-Tyrosine Kinases / genetics*
Protein-Tyrosine Kinases / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Tumor Cells, Cultured
Tyrosine / metabolism
|
| IF |
4.238
|
| 引用数 |
339
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
"SR-3Y1-1(RCB0353)(31)
3Y1-B clone 1-6 (RCB0488) |
