| RRC ID |
38781
|
| Author |
Matsumura M, Niwa Y, Hikiba Y, Okano K, Kato N, Shiina S, Shiratori Y, Omata M.
|
| Title |
Sensitive assay for detection of hepatocellular carcinoma associated gene transcription (alpha-fetoprotein mRNA) in blood.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The sensitivity of RT-PCR and nested RT-PCR for detection of circulating hepatocellular carcinoma was assessed by demonstrating the tumor cell-associated gene transcription, alpha-fetoprotein mRNA, in the nuclear cells of peripheral blood. When HepG2 cells were mixed in blood, 100-1000 tumor cells/5ml of blood could be detected by RT-PCR, in contrast to 1-10 tumor cells/5ml of blood by nested RT-PCR. In addition, 2 x 10(4) copies of AFP mRNA were found in one HepG2 cell when analyzed by the quantitative nested RT-PCR assay. Thus, the nested RT-PCR assay could provide a useful tool for detecting a tiny amount of circulating tumor cells in patients with hepatocellular carcinoma presenting extra-hepatic metastasis.
|
| Volume |
207(2)
|
| Pages |
813-8
|
| Published |
1995-2-15
|
| DOI |
10.1006/bbrc.1995.1259
|
| PII |
S0006291X85712594
|
| PMID |
7532405
|
| MeSH |
Base Sequence
Carcinoma, Hepatocellular / blood*
Cell Line
Cell Nucleus / chemistry
Cell Nucleus / metabolism
DNA Primers
Gene Expression
Humans
Liver Neoplasms / blood*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
RNA, Messenger / analysis
RNA, Messenger / blood*
Reference Values
Sensitivity and Specificity
Transcription, Genetic*
Tumor Cells, Cultured
alpha-Fetoproteins / biosynthesis*
|
| IF |
2.985
|
| Times Cited |
35
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB0459) |
