論文 - 詳細
| RRC ID | 38784 |
|---|---|
| 著者 | Kobayashi S, Kondo S, Juni K. |
| タイトル | Permeability of peptides and proteins in human cultured alveolar A549 cell monolayer. |
| ジャーナル | Pharm Res |
| Abstract |
PURPOSE:The transport of peptides or proteins across the alveolar cell monolayer was studied in vitro in order to elucidate their transport pathway. METHODS:The permeability of 14 peptides or proteins and 6 dextrans with MW 1,000-150,000 was measured in cultured human lung adenocarcinoma A549 cell monolayers at 37 degrees C or 4 degrees C. The stability of the tested peptides and proteins was also evaluated. RESULTS:The permeability coefficients of these macromolecules across the A549 cell monolayer at 37 degrees C ranged from 10(-5) to 10(-7) (cm/sec), and exhibited a good inverse correlation with molecular weight. All macromolecules were stable throughout the transport experiment, and degradation by proteases was minimal. Permeability at 4 degrees C did not differ from that at 37 degrees C. Clear selectivity for direction of transport was not observed. CONCLUSIONS:These results suggested that the tested peptides and proteins appeared to penetrate the A549 cell monolayer via a paracellular route by passive diffusion. |
| 巻・号 | 12(8) |
| ページ | 1115-9 |
| 公開日 | 1995-8-1 |
| DOI | 10.1023/a:1016295406473 |
| PMID | 7494821 |
| MeSH | Adenocarcinoma / metabolism Bradykinin / metabolism Dextrans Fluorescein-5-isothiocyanate / analogs & derivatives Humans Lung Neoplasms / metabolism Molecular Weight Peptides / chemistry Peptides / metabolism* Permeability Proteins / chemistry Proteins / metabolism* Pulmonary Alveoli / cytology Pulmonary Alveoli / metabolism* Tumor Cells, Cultured |
| IF | 3.242 |
| 引用数 | 49 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |