Reference - Detail
| RRC ID | 38794 |
|---|---|
| Author | Suzuki K, Uchii M, Nozawa R. |
| Title | Expression of calcitonin receptors on human myeloid leukemia cells. |
| Journal | J Biochem |
| Abstract |
Certain osteoclastic markers (multinucleation and tartrate-resistant acid phosphatase) were induced in human leukemia HL-60 cells by treatment with 10(-7) M 1,25-dihydroxy-vitamin D3 [1,25(OH)2D3] for 10 days. However, no formation of pits on a bone substrate by vitamin-treated HL-60 cells was detected. Expression of calcitonin receptors (CTR), another osteoclastic marker, was examined by means of the reverse transcriptase polymerase chain reaction. The human CTR-cDNA (T47D isotype) was amplified from untreated HL-60 cells, but not from cells treated with 1,25(OH)2D3. The CTR mRNA disappeared within 24 h after the treatment. Thus, 1,25(OH)2D3-differentiated HL-60 cells failed to show two intrinsic characteristics of osteoclasts, pit formation on a bone substrate and expression of CTR. We then examined the expression of CTR on established human leukemia cell lines. The CTR mRNA was expressed in myeloblastic ML-1 and promyelocytic HL-60 leukemia cells but not in more mature macrophage-like cell lines, U-937 and THP-1 cells. Neither B cell leukemia BALL-1, T cell leukemia Jurkat, promegakaryoblastic leukemia Meg-J, nor cervix uteri carcinoma HeLa S3 cells amplified the CTR products. The cDNA of BIN67-isotype CTR, that has an additional 16-amino acid insert in the putative first intracellular loop of T47D-type CTR [Kuestner et al. (1994) Mol. Pharmacol. 46, 246-255], was amplified by neither strain tested. It was suggested that the T47D-type CTR is a novel differentiation antigen of immature myeloid lineage cells. |
| Volume | 118(2) |
| Pages | 448-52 |
| Published | 1995-8-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a124928 |
| PMID | 8543584 |
| MeSH | Acid Phosphatase / biosynthesis Base Sequence Calcitriol / pharmacology Cell Nucleus DNA Primers DNA, Complementary Humans Leukemia, Myeloid / metabolism* Molecular Sequence Data Receptors, Calcitonin / biosynthesis* Tumor Cells, Cultured |
| IF | 2.476 |
| Times Cited | 5 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | BALL-1(RCB0256) |