論文 - 詳細
| RRC ID | 38799 |
|---|---|
| 著者 | Perkins SL, Kling SJ. |
| タイトル | Local concentrations of macrophage colony-stimulating factor mediate osteoclastic differentiation. |
| ジャーナル | Am J Physiol |
| Abstract |
Macrophage colony-stimulating factor (M-CSF) is essential for differentiation of osteoclasts and macrophages from a common bone marrow precursor. Using ST-2 stromal cell/murine bone marrow coculture, we studied the effects of increasing amounts of M-CSF on differentiation of macrophages and osteoclasts. Addition of exogenous M-CSF caused a dose-dependent 98% decrease in tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells, accompanied by a 2.5-fold increase in nonspecific esterase-staining macrophages. Similar decrease in osteoclastic functional activity, including 125I-labeled calcitonin binding and calcitonin-stimulated adenosine 3',5'-cyclic monophosphate (cAMP) production, were observed. Addition of exogenous M-CSF beyond 6 days in coculture had a decreasing ability to inhibit osteoclast formation, suggesting that M-CSF exerts its effects early in osteoclast differentiation, during the proposed proliferative phase of osteoclast formation. Similarly, early addition of neutralizing anti-M-CSF inhibited osteoclast formation, with diminishing effects beyond day 9. These results suggest that local high concentrations of M-CSF may influence the early determination of terminal differentiation into either macrophages or osteoclasts. |
| 巻・号 | 269(6 Pt 1) |
| ページ | E1024-30 |
| 公開日 | 1995-12-1 |
| DOI | 10.1152/ajpendo.1995.269.6.E1024 |
| PMID | 8572192 |
| MeSH | Acid Phosphatase / metabolism Animals Bone Marrow Cells Cell Differentiation / drug effects Coculture Techniques Dose-Response Relationship, Drug Esterases / metabolism Isoenzymes / metabolism Macrophage Colony-Stimulating Factor / pharmacology* Macrophages / cytology Macrophages / metabolism Mice Osmolar Concentration Osteoclasts / cytology* Stem Cells / cytology Stromal Cells / cytology Tartrate-Resistant Acid Phosphatase |
| IF | 3.485 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |