| RRC ID |
38802
|
| 著者 |
Yamada K, Harada N, Honda S, Takagi Y.
|
| タイトル |
Regulation of placenta-specific expression of the aromatase cytochrome P-450 gene. Involvement of the trophoblast-specific element binding protein.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
The aromatase (cytochrome P-450AROM) gene contains multiple untranslated exons I that are differentially transcribed in a tissue-specific manner. DNA sequences within the initial -301 upstream of placenta-specific exon I (exon Ia) are sufficient for placenta-specific expression of aromatase. In gel mobility shift assay, three separate domains in this region form specific binding complexes with proteins extracted from choriocarcinoma JEG-3 nuclei. A fragment containing these domains activates transcription driven by a heterologous promoter in a cell type-specific manner. Two of the binding domains that form major complexes in gel shift assay compete with each other and with a DNA fragment containing the trophoblast-specific element (TSE), which is derived from the enhancer region of the human chorionic gonadotropin alpha-subunit gene and is believed to confer placenta-specific expression of the gene. The core sequence RNCCTNNRG is sufficient for recognition of the TSE-binding protein, which is detected only in nuclear extracts prepared from placenta and choriocarcinoma. A mutation introduced in the distal TSE core in aromatase promoter resulted in marked reduction of transcriptional activity, although TSE region by itself did not show enhancer activity as that in human chorionic gonadotropin alpha-subunit gene.
|
| 巻・号 |
270(42)
|
| ページ |
25064-9
|
| 公開日 |
1995-10-20
|
| DOI |
10.1074/jbc.270.42.25064
|
| PII |
S0021-9258(18)87089-2
|
| PMID |
7559637
|
| MeSH |
Aromatase / genetics*
Base Sequence
Binding Sites
DNA / chemistry
DNA / metabolism
DNA-Binding Proteins / physiology*
Exons
Female
Gene Expression Regulation, Enzymologic*
Humans
Molecular Sequence Data
Placenta / enzymology*
Pregnancy
Promoter Regions, Genetic
Transcription, Genetic
|
| IF |
4.238
|
| 引用数 |
49
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2(RCB0459) |
