論文 - 詳細
| RRC ID | 38803 |
|---|---|
| 著者 | Yoshida A, Hara T, Ikenaga H, Takeuchi M. |
| タイトル | Cloning and expression of a porcine UDP-GalNAc: polypeptide N-acetylgalactosaminyl transferase. |
| ジャーナル | Glycoconj J |
| Abstract |
By employing a bovine UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyl transferase (O-GalNAc transferase) cDNA as a probe, we isolated four overlapping cDNAs from a porcine lung cDNA library. Both the nucleotide sequence of the porcine cDNA and the predicted primary structure of the protein (559 amino acids) proved to be very similar to those of the bovine enzyme (95% and 99% identity, respectively). Transient expression of the clone in COS-7 cells, followed by enzymatic activity assays, demonstrated that this cDNA sequence encodes a porcine O-GalNAc transferase. The intracellular O-GalNAc transferase activity was increased approximately 100-fold by transfecting cells with the porcine cDNA. |
| 巻・号 | 12(6) |
| ページ | 824-828 |
| 公開日 | 1995-12-1 |
| DOI | 10.1007/BF00731244 |
| PMID | 8748160 |
| MeSH | Amino Acid Sequence Animals Cell Line Cloning, Molecular* DNA, Complementary / isolation & purification* Lung / enzymology* Molecular Sequence Data N-Acetylgalactosaminyltransferases / biosynthesis N-Acetylgalactosaminyltransferases / genetics* Restriction Mapping Swine |
| IF | 2.197 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |