RRC ID 38825
Author Hassan S, Kinoshita Y, Min D, Nakata H, Kishi K, Matsushima Y, Asahara M, Wang HY, Okada A, Maekawa T, Matsui H, Chiba T.
Title Presence of prostaglandin EP4 receptor gene expression in a rat gastric mucosal cell line.
Journal Digestion
Abstract RGM-1 is an epithelial cell line established from gastric mucosa of adult Wistar rats. In this study, we characterized this newly established cell line by Northern blot analysis. We also investigated deoxyribonucleic acid (DNA) synthesis and hexosamine production in RGM-1 by PGE2. Northern blot analysis did not detect any transcript of proton pump, gastrin receptor, histidine decarboxylase, somatostatin and pepsinogen 1, indicating the absence of characteristics of parietal, ECL, D and chief cells in RGM-1 cells. However, this periodic acid-Schiff (PAS)-positive cell line expressed prostaglandin EP4 receptor mRNA but not EP1 and EP3 receptor mRNAs. [3H]-thymidine incorporation into DNA of the cells was not increased by PGE2. In contrast, PGE2 increased hexosamine content in RGM-1 cells. These results suggest that RGM-1 may be a useful model of gastric mucosal cells and that PGE2 plays a role on mucin synthesis in RGM-1 cells possibly via EP4 receptors.
Volume 57(3)
Pages 196-200
Published 1996
DOI 10.1159/000201339
PMID 8739095
MeSH Animals Blotting, Northern Cell Line DNA / biosynthesis DNA / drug effects Dinoprostone / pharmacology Gastric Mucosa / cytology Gastric Mucosa / drug effects Gastric Mucosa / metabolism* Gene Expression* Hexosamines / biosynthesis Male RNA, Messenger / metabolism* Rats Rats, Wistar Receptors, Prostaglandin E / biosynthesis* Receptors, Prostaglandin E / genetics
IF 2.037
Times Cited 67
Human and Animal Cells