RRC ID |
38827
|
著者 |
Enomoto K, Furuya K, Moore RC, Yamagishi S, Oka T, Maeno T.
|
タイトル |
Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells.
|
ジャーナル |
Biol Signals
|
Abstract |
Extracellularly applied ATP, UTP and UDP induce a transient increase in the intracellular Ca2+ concentration of mammary cells via a P2U receptor. The P2U receptor in the mammary tumor cell line MMT060562 was cloned and expressed in the human leukemia cell line K-562. The deduced amino acid sequence of the mammary tumor cell P2U receptor was 98% homologous with that of mouse NG108-15 cells. It was a member of the superfamily of GTP-binding-protein-coupled receptors. ATP and UTP induced the increase in the intracellular concentrations of Ca2+ and inositol-1,4,5-trisphosphate in both mammary tumor cells and P2U-receptor-expressed K562 cells. Dose-response curves on the production of inositol-1,4,5-trisphosphate and Ca2+ by ATP and UTP were consistently similar. Injection of GTP enhanced the ATP-induced outward current and injection of GTP gamma S induced a repetitive outward current. Both pertussis and cholera toxins did not affect ATP-induced calcium increase. It was suggested that the P2U receptor coupled with pertussis- and cholera-toxin-insensitive GTP-binding proteins and activated phosphoinositide turnover.
|
巻・号 |
5(1)
|
ページ |
9-21
|
公開日 |
1996-1-1
|
DOI |
10.1159/000109169
|
PMID |
8739319
|
MeSH |
Adenosine Diphosphate Ribose / analogs & derivatives
Adenosine Diphosphate Ribose / pharmacology
Adenosine Triphosphate / metabolism
Adenosine Triphosphate / pharmacology
Amino Acid Sequence
Animals
Base Sequence
Caffeine / pharmacology
Calcium / metabolism
Cell Line
Cloning, Molecular
Cyclic ADP-Ribose
DNA, Complementary / analysis
Female
Fluorescent Dyes
Fura-2
GTP-Binding Proteins / metabolism
Humans
Inositol 1,4,5-Trisphosphate / metabolism
Leukemia / metabolism
Mammary Neoplasms, Experimental / genetics*
Mammary Neoplasms, Experimental / metabolism
Mice
Molecular Sequence Data
Phosphatidylinositols / metabolism
Receptors, Purinergic P2 / genetics
Receptors, Purinergic P2 / physiology*
Receptors, Purinergic P2Y2
Ryanodine / pharmacology
Signal Transduction*
Suramin / pharmacology
Uridine Triphosphate / pharmacology
|
引用数 |
10
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
K562(RCB0027) |