RRC ID 38827
著者 Enomoto K, Furuya K, Moore RC, Yamagishi S, Oka T, Maeno T.
タイトル Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells.
ジャーナル Biol Signals
Abstract Extracellularly applied ATP, UTP and UDP induce a transient increase in the intracellular Ca2+ concentration of mammary cells via a P2U receptor. The P2U receptor in the mammary tumor cell line MMT060562 was cloned and expressed in the human leukemia cell line K-562. The deduced amino acid sequence of the mammary tumor cell P2U receptor was 98% homologous with that of mouse NG108-15 cells. It was a member of the superfamily of GTP-binding-protein-coupled receptors. ATP and UTP induced the increase in the intracellular concentrations of Ca2+ and inositol-1,4,5-trisphosphate in both mammary tumor cells and P2U-receptor-expressed K562 cells. Dose-response curves on the production of inositol-1,4,5-trisphosphate and Ca2+ by ATP and UTP were consistently similar. Injection of GTP enhanced the ATP-induced outward current and injection of GTP gamma S induced a repetitive outward current. Both pertussis and cholera toxins did not affect ATP-induced calcium increase. It was suggested that the P2U receptor coupled with pertussis- and cholera-toxin-insensitive GTP-binding proteins and activated phosphoinositide turnover.
巻・号 5(1)
ページ 9-21
公開日 1996-1-1
DOI 10.1159/000109169
PMID 8739319
MeSH Adenosine Diphosphate Ribose / analogs & derivatives Adenosine Diphosphate Ribose / pharmacology Adenosine Triphosphate / metabolism Adenosine Triphosphate / pharmacology Amino Acid Sequence Animals Base Sequence Caffeine / pharmacology Calcium / metabolism Cell Line Cloning, Molecular Cyclic ADP-Ribose DNA, Complementary / analysis Female Fluorescent Dyes Fura-2 GTP-Binding Proteins / metabolism Humans Inositol 1,4,5-Trisphosphate / metabolism Leukemia / metabolism Mammary Neoplasms, Experimental / genetics* Mammary Neoplasms, Experimental / metabolism Mice Molecular Sequence Data Phosphatidylinositols / metabolism Receptors, Purinergic P2 / genetics Receptors, Purinergic P2 / physiology* Receptors, Purinergic P2Y2 Ryanodine / pharmacology Signal Transduction* Suramin / pharmacology Uridine Triphosphate / pharmacology
引用数 10
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 K562(RCB0027)