Reference - Detail
RRC ID | 38833 |
---|---|
Author | Wang DS, Ueno Y, Oyamada H, Kojima S. |
Title | Accumulation of NCC-CO-411 monoclonal antibody in human colorectal adenocarcinoma through binding of the released carcinoembryonic antigen in nude mice. |
Journal | Biol Pharm Bull |
Abstract |
NCC-CO-411, an anti-carcinoembryonic antigen (CEA) monoclonal antibody (MoAb) showed a dose-dependent reactivity with standard CEA, but did not bind to the CEA anchoring with cell membranes. When LS 174T cells, one of the CEA-producing cells, were treated with phosphatidylinositol-specific phospholipase C, NCC-CO-411 MoAb recognized the released CEA containing glycosylphosphatidylinositol as well as the standard CEA. The binding activity of NCC-CO-411 MoAb with CEA was also demonstrated by the immunostaining of the LS 174T colorectal tumor xenograft tissues. The biodistribution study showed that NCC-CO-411 MoAb was mainly taken up by tumor and kidney, while the anti-hCEA MoAb was largely concentrated in liver and spleen rather than in the tumor. These results suggest that NCC-CO-411 MoAb recognizes the released CEA and gives an excellent tumor image in LS 174T tumor-bearing mice. |
Volume | 19(7) |
Pages | 915-21 |
Published | 1996-7-1 |
DOI | 10.1248/bpb.19.915 |
PMID | 8839961 |
MeSH | Adenocarcinoma / metabolism* Adenocarcinoma / pathology Animals Antibodies, Monoclonal / metabolism* Antibody Specificity Carcinoembryonic Antigen / metabolism* Colorectal Neoplasms / metabolism* Colorectal Neoplasms / pathology Cross Reactions Enzyme-Linked Immunosorbent Assay Humans Immunohistochemistry Indium Radioisotopes Iodine Radioisotopes Mice Mice, Nude Radioimmunoassay Tissue Distribution |
IF | 1.863 |
Times Cited | 0 |
WOS Category | PHARMACOLOGY & PHARMACY |
Resource | |
Human and Animal Cells | GCIY(RCB0555) HGC-27(RCB0500) |