論文 - 詳細
| RRC ID | 38842 |
|---|---|
| 著者 | Yamaguchi N, Yamashita Y, Ikeda D, Koga T. |
| タイトル | Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide antigen stimulates production of chemotactic factors and inflammatory cytokines by human monocytes. |
| ジャーナル | Infect Immun |
| Abstract |
Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1alpha, IL-1beta, IL-6, and TNF alpha (TNF-alpha) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1alpha, I1-1beta, IL-6, and TNF-alpha, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes. |
| 巻・号 | 64(7) |
| ページ | 2563-70 |
| 公開日 | 1996-7-1 |
| DOI | 10.1128/iai.64.7.2563-2570.1996 |
| PMID | 8698480 |
| PMC | PMC174111 |
| MeSH | Aggregatibacter actinomycetemcomitans / classification Aggregatibacter actinomycetemcomitans / immunology* Antigens, Bacterial* / isolation & purification Antigens, Bacterial* / pharmacology Base Sequence Chemokine CCL2 / biosynthesis Chemokine CCL2 / genetics Chemotactic Factors / biosynthesis* Chemotactic Factors / genetics Cytokines / biosynthesis* Cytokines / genetics DNA Primers / genetics Gene Expression Humans In Vitro Techniques Interleukin-1 / biosynthesis Interleukin-1 / genetics Interleukin-6 / biosynthesis Interleukin-6 / genetics Interleukin-8 / biosynthesis Interleukin-8 / genetics Molecular Sequence Data Monocytes / immunology* Monocytes / metabolism Polysaccharides, Bacterial / immunology* Polysaccharides, Bacterial / isolation & purification Polysaccharides, Bacterial / pharmacology RNA, Messenger / genetics RNA, Messenger / metabolism Serotyping Tumor Necrosis Factor-alpha / biosynthesis Tumor Necrosis Factor-alpha / genetics |
| IF | 3.201 |
| 引用数 | 24 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB0081) Hep G2(RCB0459) |