論文 - 詳細
| RRC ID | 38855 |
|---|---|
| 著者 | Tsuji S, Kawano S, Sawaoka H, Takei Y, Kobayashi I, Nagano K, Fusamoto H, Kamada T. |
| タイトル | Evidences for involvement of cyclooxygenase-2 in proliferation of two gastrointestinal cancer cell lines. |
| ジャーナル | Prostaglandins Leukot Essent Fatty Acids |
| Abstract |
Cyclooxygenase (COX) consists of two isozymes, COX-1 and COX-2. The roles of these isozymes in the gastrointestinal tract are unknown. We investigated messenger RNA expression of the COX-1 and COX-2 genes in the gastrointestinal cancer cell lines MKN28, MKN45, KATO III CACO-2, DLD-1 and LoVo. These cell lines expressed comparable levels of COX-1 mRNA, although their expression of COX-2 varied. Therefore, we studied the effects of NS-398 and indomethacin, specific and non-specific inhibitors for COX-2, on proliferation of the cell lines. Both of the inhibitors suppressed proliferation of the two cell lines that highly expressed COX-2 (MKN45 and CACO-2). However, these inhibitors exerted minimal effects on proliferation of the other cell lines, which expressed significantly lower levels of COX-2. Therefore, it was proposed that COX-2 participates in proliferation of cancer cells because of over expression of the COX-2 gene. |
| 巻・号 | 55(3) |
| ページ | 179-83 |
| 公開日 | 1996-9-1 |
| DOI | 10.1016/s0952-3278(96)90095-2 |
| PII | S0952-3278(96)90095-2 |
| PMID | 8931116 |
| MeSH | Cell Division / drug effects* Cell Line Colonic Neoplasms / enzymology Cyclooxygenase 1 Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cyclooxygenase Inhibitors / pharmacology* Gastrointestinal Neoplasms / enzymology* Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Indomethacin / pharmacology Isoenzymes / drug effects* Isoenzymes / genetics Membrane Proteins Nitrobenzenes / pharmacology Prostaglandin-Endoperoxide Synthases / drug effects* Prostaglandin-Endoperoxide Synthases / genetics RNA, Messenger / analysis Stomach Neoplasms / enzymology Sulfonamides / pharmacology Tumor Cells, Cultured |
| IF | 2.932 |
| 引用数 | 104 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) DLD-1(RCB1957) LoVo(RCB1639) |