| RRC ID |
38856
|
| 著者 |
Niwa Y, Matsumura M, Shiratori Y, Imamura M, Kato N, Shiina S, Okudaira T, Ikeda Y, Inoue T, Omata M.
|
| タイトル |
Quantitation of alpha-fetoprotein and albumin messenger RNA in human hepatocellular carcinoma.
|
| ジャーナル |
Hepatology
|
| Abstract |
To analyze gene expression of alpha-fetoprotein (AFP) and albumin in hepatocellular carcinoma (HCC), messenger RNAs (mRNAs) of these proteins in six human hepatoma cell lines and in 30 cases of HCC were quantitatively analyzed by competitive reverse transcription (RT) followed by polymerase chain reaction (PCR). The transcriptional levels of both AFP and albumin genes in HepG2 and Huh 7 cell lines were 10(10) copies/microgram RNA, in contrast to approximately 10(5) copies/microgram RNA in HLE and HLF cell lines. AFP and albumin mRNA levels in three normal livers were 10(5) and 10(10) transcripts/microgram RNA, respectively. In 30 cases with HCC AFP mRNA level in neoplasm was 10 to 10(5)-fold enhanced as compared with that of nonneoplastic portion, and correlated with serum AFP level and tumor size (P < .01). In contrast, albumin mRNA level was not reduced in the neoplasms presenting enhanced AFP mRNA levels, indicating that AFP and albumin gene expression in situ is not necessarily mutually exclusive. Prospective analysis revealed that an increased serum AFP was shown at the time of recurrence among patients with enhanced AFP mRNA levels in neoplasm only, indicating that AFP mRNA levels in neoplasm could be a clinically predictable tool.
|
| 巻・号 |
23(6)
|
| ページ |
1384-92
|
| 公開日 |
1996-6-1
|
| DOI |
10.1053/jhep.1996.v23.pm0008675155
|
| PII |
S0270-9139(96)00225-X
|
| PMID |
8675155
|
| MeSH |
Aged
Albumins / genetics*
Base Sequence
Carcinoma, Hepatocellular / chemistry*
Carcinoma, Hepatocellular / genetics*
Carcinoma, Hepatocellular / pathology
DNA Primers / genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Liver Neoplasms / chemistry*
Liver Neoplasms / genetics*
Liver Neoplasms / pathology
Male
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / statistics & numerical data
RNA, Messenger / analysis*
RNA, Messenger / genetics*
RNA, Neoplasm / analysis*
RNA, Neoplasm / genetics*
Recurrence
Reproducibility of Results
Tumor Cells, Cultured
alpha-Fetoproteins / genetics*
|
| IF |
14.679
|
| 引用数 |
54
|
|
WOS 分野
|
GASTROENTEROLOGY & HEPATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2(RCB459) |
