RRC ID 3886
著者 Koyama T, Iwamuro S.
タイトル Molecular cloning of a cDNA encoding atypical antimicrobial and cytotoxic Brevinin-1Ja from the skin of the Japanese brown frog, Rana japonica.
ジャーナル Zoolog Sci
Abstract Antimicrobial peptides (AMPs) are important components of the host innate defense system against pathogenic microbial invasion in many organisms. In the present study, we cloned by RT-PCR a cDNA from total RNA prepared from the skin of the Japanese brown frog Rana japonica. The cDNA directs the synthesis of a novel, non-C-terminally alpha-amidated peptide composed of 21 amino acid residues (FLGSLIGAAIPAIKQLLGLKK). The putative peptide showed limited sequence similarity to atypical acyclic brevinin-1OK family AMPs originally isolated from the skin of the Ryukyu brown frog (R. okinavana), which lacks the COOH-terminal cyclic domain commonly observed in typical brevinin-1 groups, but that contains a C-terminally alpha-amidated residue. Although it is unclear whether the peptide, designated brevinin-1Ja, is produced in R. japonica skin, a synthetic replicate of the peptide showed differential growth-inhibiting activity against the Gram-positive bacterium Staphylococcus aureus and Gram-negative bacterium Escherichia coli (minimal inhibitory concentrations: 15 microM and 119 microM, respectively), and produced cell death of mammalian COS7 cells (LD50=28 microM).
巻・号 25(5)
ページ 487-91
公開日 2008-5-1
DOI 10.2108/zsj.25.487
PII 0289-0003-25-5-487
PMID 18558801
MeSH Amino Acid Sequence Amphibian Proteins / genetics* Animals Antimicrobial Cationic Peptides / genetics* Antineoplastic Agents / metabolism Base Sequence Cloning, Molecular DNA, Complementary / genetics* Molecular Sequence Data Ranidae / genetics* Skin / metabolism*
IF 0.843
引用数 8
WOS 分野 ZOOLOGY
リソース情報
ヒト・動物細胞 COS-7(RCB0539)
一般微生物 JCM2874 JCM5491