| RRC ID |
38864
|
| Author |
Clohisy DR, Ogilvie CM, Carpenter RJ, Ramnaraine ML.
|
| Title |
Localized, tumor-associated osteolysis involves the recruitment and activation of osteoclasts.
|
| Journal |
J Orthop Res
|
| Abstract |
The cellular and biochemical mechanisms that direct destruction of bone at the site of tumor osteolysis are unknown. In order to understand this process better, a murine model designed for the study of tumor osteolysis was developed and the influence of osteolytic and nonosteolytic tumors on bone was investigated. Tumors developed following femoral intramedullary injection of sarcoma (2472) and melanoma (G3.26) cell lines; however, only tumors from the 2472 cell line caused osteolysis. It was determined that 2472 tumor-induced osteolysis commenced 6 days after the femora had been inoculated with 2472 cells. There were more osteoclasts per millimeter of bone surface in 2472 tumor-bearing limbs (16.7 +/- 5.0) than in sham-injected limbs (3.8 +/- 0.9) (p < 0.015). In addition, an increase in the osteoclast size (area) was detected in 2472 tumor-bearing limbs: 412 +/- 65 micron2 compared with 187 +/- 17 micron2 (p < 0.01). In vitro bone resorption experiments indicated that 2472 tumor cells had a limited ability to destroy bone in comparison with macrophages and osteoclasts. Taken in total, these findings define a model that is useful for the study of tumor osteolysis, and the data from analyses of the model demonstrate that the cellular mechanisms responsible for 2472 tumor-induced osteolysis include both an increase in the number of osteoclasts and activation of mature osteoclasts.
|
| Volume |
14(1)
|
| Pages |
2-6
|
| Published |
1996-1-1
|
| DOI |
10.1002/jor.1100140103
|
| PMID |
8618161
|
| MeSH |
Animals
Bone Neoplasms / diagnostic imaging
Bone Neoplasms / physiopathology*
Bone Resorption
Calcium / blood
Femur
Melanoma, Experimental / diagnostic imaging
Melanoma, Experimental / physiopathology*
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Osteoclasts / physiology*
Osteolysis*
Radiography
Sarcoma, Experimental / diagnostic imaging
Sarcoma, Experimental / physiopathology*
Tumor Cells, Cultured / physiology
Tumor Cells, Cultured / transplantation
|
| IF |
2.728
|
| Times Cited |
60
|
|
WOS Category
|
ORTHOPEDICS
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224) |
