Reference - Detail
| RRC ID | 38869 |
|---|---|
| Author | Nishide Y, Tousen Y, Tadaishi M, Inada M, Miyaura C, Kruger MC, Ishimi Y. |
| Title | Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation. |
| Journal | Int J Environ Res Public Health |
| Abstract |
Soy isoflavones, genistein, daidzein and its metabolite equol, as well as β-carotene have been reported to be effective for maintaining bone health. However, it remains to be elucidated whether combining soy isoflavones with β-carotene is beneficial to bone formation. This study investigated the combined effect of soy isoflavones and β-carotene on the differentiation of MC3T3-E1 preosteoblastic cells. Daidzein and genistein alone did not affect cell growth but increased alkaline phosphatase (ALP) activity. Beta-carotene alone inhibited cell growth and markedly enhanced ALP activity. Soy isoflavones combined with β-carotene resulted in higher ALP activity than treatment with isoflavones or β-carotene alone. We observed significant main effects of β-carotene on the enhanced expression of Runx2, ALP, and ostepontin mRNA, whereas there was a significant main effect of soy isoflavones on the expression of osterix mRNA. To investigate how β-carotene affected osteoblast differentiation, MC3T3-E1 cells were treated with retinoic acid receptor (RAR) pan-antagonist combined with β-carotene. Osteopontin and ALP mRNA expression levels, which were increased following treatment with β-carotene, were significantly suppressed by the RAR pan-antagonist. This suggests treatment with β-carotene enhanced early osteoblastic differentiation, at least in part via RAR signaling. These results indicate that a combination of isoflavones and β-carotene may be useful for maintaining a positive balance of bone turnover by inducing osteoblast differentiation. |
| Volume | 12(11) |
| Pages | 13750-61 |
| Published | 2015-10-28 |
| DOI | 10.3390/ijerph121113750 |
| PII | ijerph121113750 |
| PMID | 26516892 |
| PMC | PMC4661612 |
| MeSH | Animals Cell Differentiation / drug effects* Cell Line Cell Proliferation / drug effects Drug Synergism Functional Food Isoflavones / pharmacology* Isoflavones / therapeutic use Mice Osteoblasts / drug effects* Osteoporosis / prevention & control Phytotherapy RNA, Messenger / metabolism Receptors, Retinoic Acid / antagonists & inhibitors Signal Transduction Soybeans beta Carotene / pharmacology* beta Carotene / therapeutic use |
| IF | 2.849 |
| Times Cited | 13 |
| WOS Category | PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH ENVIRONMENTAL SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |