| RRC ID |
38887
|
| Author |
Okada T, Uto K, Aoyagi T, Ebara M.
|
| Title |
A biomimetic approach to hormone resistant prostate cancer cell isolation using inactivated Sendai virus (HVJ-E).
|
| Journal |
Biomater Sci
|
| Abstract |
Our study reports a versatile immobilization method of Hemagglutinating Virus of Japan Envelope (HVJ-E) for the generation of viral-mimetic surfaces for hormone resistant prostate cancer cell isolation. HVJ-E has recently attracted much attention as a new type of therapeutic material because hormone resistant prostate cancer cells such as PC-3 cells possess the HVJ-E receptors, GD1a. The HVJ-E was successfully immobilized on precursor films composed of poly-l-lysine and alginic acid via layer-by-layer assembly without changing the biological activity. The monolayer adsorption of HVJ-E particles was confirmed by quartz crystal microbalance, fluorescent and atomic force microscopy analyses. By developing the HVJ-E coating with an affinity based cell trap within a glass capillary tube, we are able to gently isolate PC-3 from LN-Cap cells that represent adenocarcinoma without compromising cell viability. We achieved approximately 100% cell separation efficiency only by 60 seconds of flowing. We believe that the proposed technique offers significant promise for the creation of a hormone resistant cancer cell trap on a broad range of materials.
|
| Volume |
4(1)
|
| Pages |
96-103
|
| Published |
2016-1-1
|
| DOI |
10.1039/c5bm00375j
|
| PMID |
26553555
|
| MeSH |
Biomimetics / methods*
Cell Line, Tumor
Cell Separation
Cell Survival
Humans
Lysine / chemistry*
Lysine / metabolism
Male
Oncolytic Virotherapy / methods*
Prostatic Neoplasms / chemistry*
Prostatic Neoplasms / pathology
Sendai virus / chemistry*
Sendai virus / metabolism
|
| IF |
6.183
|
| Times Cited |
1
|
|
WOS Category
|
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
PC-3(RCB2145)
LNCap.FGC(RCB2144) |
