Reference - Detail
| RRC ID | 38887 |
|---|---|
| Author | Okada T, Uto K, Aoyagi T, Ebara M. |
| Title | A biomimetic approach to hormone resistant prostate cancer cell isolation using inactivated Sendai virus (HVJ-E). |
| Journal | Biomater Sci |
| Abstract |
Our study reports a versatile immobilization method of Hemagglutinating Virus of Japan Envelope (HVJ-E) for the generation of viral-mimetic surfaces for hormone resistant prostate cancer cell isolation. HVJ-E has recently attracted much attention as a new type of therapeutic material because hormone resistant prostate cancer cells such as PC-3 cells possess the HVJ-E receptors, GD1a. The HVJ-E was successfully immobilized on precursor films composed of poly-l-lysine and alginic acid via layer-by-layer assembly without changing the biological activity. The monolayer adsorption of HVJ-E particles was confirmed by quartz crystal microbalance, fluorescent and atomic force microscopy analyses. By developing the HVJ-E coating with an affinity based cell trap within a glass capillary tube, we are able to gently isolate PC-3 from LN-Cap cells that represent adenocarcinoma without compromising cell viability. We achieved approximately 100% cell separation efficiency only by 60 seconds of flowing. We believe that the proposed technique offers significant promise for the creation of a hormone resistant cancer cell trap on a broad range of materials. |
| Volume | 4(1) |
| Pages | 96-103 |
| Published | 2016-1-1 |
| DOI | 10.1039/c5bm00375j |
| PMID | 26553555 |
| MeSH | Biomimetics / methods* Cell Line, Tumor Cell Separation Cell Survival Humans Lysine / chemistry* Lysine / metabolism Male Oncolytic Virotherapy / methods* Prostatic Neoplasms / chemistry* Prostatic Neoplasms / pathology Sendai virus / chemistry* Sendai virus / metabolism |
| IF | 6.183 |
| Times Cited | 1 |
| WOS Category | MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-3(RCB2145) LNCap.FGC(RCB2144) |