| RRC ID |
38894
|
| 著者 |
Kobayashi M, Kim SH, Nakamura H, Kaneda S, Fujii T.
|
| タイトル |
Cancer Cell Analyses at the Single Cell-Level Using Electroactive Microwell Array Device.
|
| ジャーナル |
PLoS One
|
| Abstract |
Circulating tumor cells (CTCs), shed from primary tumors and disseminated into peripheral blood, are playing a major role in metastasis. Even after isolation of CTCs from blood, the target cells are mixed with a population of other cell types. Here, we propose a new method for analyses of cell mixture at the single-cell level using a microfluidic device that contains arrayed electroactive microwells. Dielectrophoretic (DEP) force, induced by the electrodes patterned on the bottom surface of the microwells, allows efficient trapping and stable positioning of single cells for high-throughput biochemical analyses. We demonstrated that various on-chip analyses including immunostaining, viability/apoptosis assay and fluorescent in situ hybridization (FISH) at the single-cell level could be conducted just by applying specific reagents for each assay. Our simple method should greatly help discrimination and analysis of rare cancer cells among a population of blood cells.
|
| 巻・号 |
10(11)
|
| ページ |
e0139980
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pone.0139980
|
| PII |
PONE-D-15-39402
|
| PMID |
26558904
|
| PMC |
PMC4641639
|
| MeSH |
Apoptosis
Cell Line, Tumor
Feasibility Studies
Humans
In Situ Hybridization, Fluorescence
Microfluidic Analytical Techniques / instrumentation*
Neoplasms / pathology*
Single-Cell Analysis*
|
| IF |
2.74
|
| 引用数 |
12
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| ヒト・動物細胞 |
U937
DU145(RCB2143)
PC-3(RCB2145) |
