論文 - 詳細
| RRC ID | 38909 |
|---|---|
| 著者 | Ando Y, Oku T, Tsuji T. |
| タイトル | Platelets attenuate production of cytokines and nitric oxide by macrophages in response to bacterial endotoxin. |
| ジャーナル | Platelets |
| Abstract |
Considerable evidence has been accumulated concerning the roles of platelets in immune responses. In the present study, we examined the functional modulation of macrophages by platelets. When mouse bone marrow-derived macrophages (BMDMs) were co-cultured with platelets, BMDMs produced lower levels of nitric oxide (NO), tumor necrosis factor-α (TNF)-α, and interleukin (IL)-6 in response to a bacterial endotoxin (LPS) and zymosan. The attenuation in the macrophage susceptibility to LPS appeared to be mediated by soluble factors secreted from platelets. The mRNA levels of NOS2 (iNOS), TNF-α, and IL-6 in LPS-stimulated BMDMs that had been cultured with a conditioned medium of platelets were also decreased as analyzed by RT-qPCR. The ability of the platelet-conditioned medium to suppress macrophage NO production was recovered in a high-molecular-weight fraction (>670 kDa) after gel-filtration chromatography on a Superose 6 column. These results suggest that platelets control the susceptibility of macrophages to prevent excessive responses to LPS and provide mechanistic insight into a previous report that experimental thrombocytopenia aggravated organ failure in LPS-induced endotoxemia. |
| 巻・号 | 27(4) |
| ページ | 344-50 |
| 公開日 | 2016-6-1 |
| DOI | 10.3109/09537104.2015.1103369 |
| PMID | 26588084 |
| MeSH | Animals Blood Platelets / metabolism* Cell Line Culture Media, Conditioned / pharmacology Cytokines / biosynthesis* Endotoxins / metabolism* Humans Lipopolysaccharides / immunology Macrophage Activation / immunology Macrophages / drug effects Macrophages / immunology* Macrophages / metabolism* Mice Nitric Oxide / biosynthesis* Thrombin / pharmacology Zymosan / immunology |
| IF | 3.378 |
| 引用数 | 17 |
| WOS 分野 | HEMATOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB2619) |