| RRC ID |
38914
|
| Author |
Hagiwara K, Katsuda T, Gailhouste L, Kosaka N, Ochiya T.
|
| Title |
Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles.
|
| Journal |
FEBS Lett
|
| Abstract |
Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray analysis revealed that ANXA2 regulated the loading of miRNAs into EVs in a sequence independent manner. Lastly, immunoprecipitation analysis confirmed that ANXA2 could bind miRNAs in EVs in the presence of Ca(2+). These observations demonstrate that ANXA2 plays an important role in the packaging process of miRNAs into EVs.
|
| Volume |
589(24 Pt B)
|
| Pages |
4071-8
|
| Published |
2015-12-21
|
| DOI |
10.1016/j.febslet.2015.11.036
|
| PII |
S0014-5793(15)01049-2
|
| PMID |
26632510
|
| MeSH |
Annexin A2 / antagonists & inhibitors
Annexin A2 / genetics
Annexin A2 / metabolism*
Annexin A5 / antagonists & inhibitors
Annexin A5 / genetics
Annexin A5 / metabolism
Biological Transport
Calcium Signaling
Cell Line, Tumor
Extracellular Vesicles / metabolism*
Gene Expression Regulation, Neoplastic
Humans
Immunoprecipitation
MicroRNAs / chemistry
MicroRNAs / metabolism*
Neoplasm Proteins / antagonists & inhibitors
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Oligonucleotide Array Sequence Analysis
Particle Size
Proteomics / methods
RNA Interference
RNA, Neoplasm / chemistry
RNA, Neoplasm / metabolism*
RNA, Small Interfering
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / metabolism
Up-Regulation*
|
| IF |
3.057
|
| Times Cited |
27
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
HuH-7 |
