Reference - Detail
| RRC ID | 38914 |
|---|---|
| Author | Hagiwara K, Katsuda T, Gailhouste L, Kosaka N, Ochiya T. |
| Title | Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles. |
| Journal | FEBS Lett |
| Abstract |
Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray analysis revealed that ANXA2 regulated the loading of miRNAs into EVs in a sequence independent manner. Lastly, immunoprecipitation analysis confirmed that ANXA2 could bind miRNAs in EVs in the presence of Ca(2+). These observations demonstrate that ANXA2 plays an important role in the packaging process of miRNAs into EVs. |
| Volume | 589(24 Pt B) |
| Pages | 4071-8 |
| Published | 2015-12-21 |
| DOI | 10.1016/j.febslet.2015.11.036 |
| PII | S0014-5793(15)01049-2 |
| PMID | 26632510 |
| MeSH | Annexin A2 / antagonists & inhibitors Annexin A2 / genetics Annexin A2 / metabolism* Annexin A5 / antagonists & inhibitors Annexin A5 / genetics Annexin A5 / metabolism Biological Transport Calcium Signaling Cell Line, Tumor Extracellular Vesicles / metabolism* Gene Expression Regulation, Neoplastic Humans Immunoprecipitation MicroRNAs / chemistry MicroRNAs / metabolism* Neoplasm Proteins / antagonists & inhibitors Neoplasm Proteins / genetics Neoplasm Proteins / metabolism* Oligonucleotide Array Sequence Analysis Particle Size Proteomics / methods RNA Interference RNA, Neoplasm / chemistry RNA, Neoplasm / metabolism* RNA, Small Interfering Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / metabolism Up-Regulation* |
| IF | 3.057 |
| Times Cited | 27 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HuH-7 |