| RRC ID |
38918
|
| Author |
Ezawa K, Minato K, Dobashi K.
|
| Title |
Induction of apoptosis by ubenimex (Bestatin) in human non-small-cell lung cancer cell lines.
|
| Journal |
Biomed Pharmacother
|
| Abstract |
We studied the direct anti-tumor effects of ubenimex on five human lung cancer cell lines; ABC-1, RERF-LC-OK, RERF-LC-MS (adenocarcinoma) and SQ-5, EBC-1 (squamous cell carcinoma). Ubenimex dose-dependently inhibited the growth of these cancer cell lines except RERF-LC-MS. The results indicated that lung squamous cell carcinoma cell lines were more sensitive to ubenimex than lung adenocarcinoma cell lines. Coincidentally, histological observation by Hematoxylin eosine (HE) staining revealed that ubenimex induced nuclear condensation and apoptic body in the cancer cell lines. Immunohistochemical study showed ubenimex-treated cells expressed LeY antigen which is a useful phenotypic marker predictive of apoptosis. The induction of DNA fragmentation was also observed in the ubenimex treated cancer cell lines by ELISA. We conclude that ubenimex exhibits its direct anti-tumor effect against non-small-cell lung cancer cell lines, more effectively against squamous carcinoma cell lines, through the induction of apoptosis.
|
| Volume |
50(6-7)
|
| Pages |
283-9
|
| Published |
1996-1-1
|
| DOI |
10.1016/0753-3322(96)84827-x
|
| PII |
0753-3322(96)84827-X
|
| PMID |
8952869
|
| MeSH |
Adenocarcinoma / immunology
Adenocarcinoma / pathology*
Antibiotics, Antineoplastic / pharmacology*
Apoptosis / drug effects*
Carcinoma, Squamous Cell / immunology
Carcinoma, Squamous Cell / pathology*
Cell Division / drug effects
DNA Fragmentation / drug effects
Humans
Leucine / analogs & derivatives*
Leucine / pharmacology
Lung Neoplasms / immunology
Lung Neoplasms / pathology*
Tumor Cells, Cultured / immunology
Tumor Cells, Cultured / pathology
Tumor Cells, Cultured / physiology
|
| IF |
4.545
|
| Times Cited |
30
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
MEDICINE, RESEARCH & EXPERIMENTAL
|
| Resource |
| Human and Animal Cells |
SQ-5(RCB0110) |
