論文 - 詳細
| RRC ID | 38919 |
|---|---|
| 著者 | Miyazaki Y, Shinomura Y, Tsutsui S, Yasunaga Y, Zushi S, Higashiyama S, Taniguchi N, Matsuzawa Y. |
| タイトル | Oxidative stress increases gene expression of heparin-binding EGF-like growth factor and amphiregulin in cultured rat gastric epithelial cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We investigated the effects of oxidative stress on mRNA levels of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AR) in rat gastric epithelial RGM1 cells. In response to stimulation with hydrogen peroxide (100-400 microM), gene expression of HB-EGF and AR increased in a dose-dependent manner, peaked at 3 h, and returned to the base line at 7 h. Hydrogen peroxide-induced HB-EGF and AR gene expression was blocked by pretreatment with an antioxidant N-acetyl-cysteine. In addition, it was significantly inhibited by pretreatment with EGF receptor-specific tyrphostin AG1478, but not by depletion of protein kinase C. These data indicate that oxidative stress upregulates expression of EGF-related polypeptides and the possible involvement of EGF receptor in this process. |
| 巻・号 | 226(2) |
| ページ | 542-6 |
| 公開日 | 1996-9-13 |
| DOI | 10.1006/bbrc.1996.1391 |
| PII | S0006-291X(96)91391-1 |
| PMID | 8806670 |
| MeSH | Acetylcysteine / pharmacology Amphiregulin Animals Cell Line EGF Family of Proteins Epidermal Growth Factor / genetics* Gastric Mucosa / drug effects Gastric Mucosa / enzymology Gastric Mucosa / metabolism* Gene Expression Regulation* / drug effects Glycoproteins / genetics* Growth Substances / genetics* Heparin-binding EGF-like Growth Factor Hydrogen Peroxide / pharmacology Intercellular Signaling Peptides and Proteins* Oxidative Stress* Protein Kinase C / antagonists & inhibitors Protein-Tyrosine Kinases / antagonists & inhibitors RNA, Messenger / genetics RNA, Messenger / metabolism Rats |
| IF | 2.985 |
| 引用数 | 41 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |