RRC ID |
38919
|
著者 |
Miyazaki Y, Shinomura Y, Tsutsui S, Yasunaga Y, Zushi S, Higashiyama S, Taniguchi N, Matsuzawa Y.
|
タイトル |
Oxidative stress increases gene expression of heparin-binding EGF-like growth factor and amphiregulin in cultured rat gastric epithelial cells.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
We investigated the effects of oxidative stress on mRNA levels of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AR) in rat gastric epithelial RGM1 cells. In response to stimulation with hydrogen peroxide (100-400 microM), gene expression of HB-EGF and AR increased in a dose-dependent manner, peaked at 3 h, and returned to the base line at 7 h. Hydrogen peroxide-induced HB-EGF and AR gene expression was blocked by pretreatment with an antioxidant N-acetyl-cysteine. In addition, it was significantly inhibited by pretreatment with EGF receptor-specific tyrphostin AG1478, but not by depletion of protein kinase C. These data indicate that oxidative stress upregulates expression of EGF-related polypeptides and the possible involvement of EGF receptor in this process.
|
巻・号 |
226(2)
|
ページ |
542-6
|
公開日 |
1996-9-13
|
DOI |
10.1006/bbrc.1996.1391
|
PII |
S0006-291X(96)91391-1
|
PMID |
8806670
|
MeSH |
Acetylcysteine / pharmacology
Amphiregulin
Animals
Cell Line
EGF Family of Proteins
Epidermal Growth Factor / genetics*
Gastric Mucosa / drug effects
Gastric Mucosa / enzymology
Gastric Mucosa / metabolism*
Gene Expression Regulation* / drug effects
Glycoproteins / genetics*
Growth Substances / genetics*
Heparin-binding EGF-like Growth Factor
Hydrogen Peroxide / pharmacology
Intercellular Signaling Peptides and Proteins*
Oxidative Stress*
Protein Kinase C / antagonists & inhibitors
Protein-Tyrosine Kinases / antagonists & inhibitors
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
|
IF |
2.985
|
引用数 |
41
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
RGM1(RCB0876) |