RRC ID 38919
著者 Miyazaki Y, Shinomura Y, Tsutsui S, Yasunaga Y, Zushi S, Higashiyama S, Taniguchi N, Matsuzawa Y.
タイトル Oxidative stress increases gene expression of heparin-binding EGF-like growth factor and amphiregulin in cultured rat gastric epithelial cells.
ジャーナル Biochem Biophys Res Commun
Abstract We investigated the effects of oxidative stress on mRNA levels of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AR) in rat gastric epithelial RGM1 cells. In response to stimulation with hydrogen peroxide (100-400 microM), gene expression of HB-EGF and AR increased in a dose-dependent manner, peaked at 3 h, and returned to the base line at 7 h. Hydrogen peroxide-induced HB-EGF and AR gene expression was blocked by pretreatment with an antioxidant N-acetyl-cysteine. In addition, it was significantly inhibited by pretreatment with EGF receptor-specific tyrphostin AG1478, but not by depletion of protein kinase C. These data indicate that oxidative stress upregulates expression of EGF-related polypeptides and the possible involvement of EGF receptor in this process.
巻・号 226(2)
ページ 542-6
公開日 1996-9-13
DOI 10.1006/bbrc.1996.1391
PII S0006-291X(96)91391-1
PMID 8806670
MeSH Acetylcysteine / pharmacology Amphiregulin Animals Cell Line EGF Family of Proteins Epidermal Growth Factor / genetics* Gastric Mucosa / drug effects Gastric Mucosa / enzymology Gastric Mucosa / metabolism* Gene Expression Regulation* / drug effects Glycoproteins / genetics* Growth Substances / genetics* Heparin-binding EGF-like Growth Factor Hydrogen Peroxide / pharmacology Intercellular Signaling Peptides and Proteins* Oxidative Stress* Protein Kinase C / antagonists & inhibitors Protein-Tyrosine Kinases / antagonists & inhibitors RNA, Messenger / genetics RNA, Messenger / metabolism Rats
IF 2.985
引用数 41
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RGM1(RCB0876)