RRC ID |
38944
|
著者 |
Kitajima I, Kawahara K, Hanyu N, Shin H, Tokioka T, Soejima Y, Tsutsui J, Ozawa M, Shimayama T, Maruyama I.
|
タイトル |
Enhanced E-cadherin expression and increased calcium-dependent cell-cell adhesion in human T-cell leukemia virus type I Tax-expressing PC12 cells.
|
ジャーナル |
J Cell Sci
|
Abstract |
Human T-cell leukemia virus type I (HTLV-I) Tax protein induces the expression of host cellular genes, some of which are crucial in cell proliferation and differentiation. We examined the mechanisms by which HTLV-I Tax protein induces phenotypic changes in PC12 cells. We demonstrated that the HTLV-I Tax gene induces epithelioid changes and increases cell-cell contact in PC12 cells. No change in the expression of the neural cell adhesion molecule was observed between HTLV-I Tax-expressing PC12 cells and PC12 cells transfected with a control plasmid. However, HTLV-I Tax-expressing PC12 cells demonstrated a marked change in the abundance and distribution of E-cadherin, which was concentrated at regions of cellular contact and accompanied by changes in calcium-dependent cell adhesion. Although E-cadherin is expressed at low levels in PC12 and PC12 transfected with a control plasmid cells, the steady state level of E-cadherin in tax-expressing PC12 cells increases significantly, apparently as a result of regulation at the transcriptional level. Diminished expression of Tax protein in Tax-expressing PC12 cells exposed to antisense oligonucleotides for the Tax gene suppresses E-cadherin expression and decreases cell-cell adhesion. These findings imply that HTLV-I Tax protein enhanced E-cadherin expression modulates calcium-dependent cell-cell adhesion mechanisms.
|
巻・号 |
109 ( Pt 3)
|
ページ |
609-17
|
公開日 |
1996-3-1
|
DOI |
10.1242/jcs.109.3.609
|
PMID |
8907706
|
MeSH |
Animals
Base Sequence
Cadherins / biosynthesis*
Calcium / physiology*
Cell Adhesion / physiology*
Gene Products, tax / biosynthesis*
Human T-lymphotropic virus 1*
Molecular Sequence Data
Oligonucleotides, Antisense
PC12 Cells
RNA, Messenger / biosynthesis
Rats
|
IF |
4.573
|
引用数 |
13
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
PC-12(RCB0009) |