| RRC ID |
38945
|
| 著者 |
Matsuzawa A, Murakami M, Atsumi G, Imai K, Prados P, Inoue K, Kudo I.
|
| タイトル |
Release of secretory phospholipase A2 from rat neuronal cells and its possible function in the regulation of catecholamine secretion.
|
| ジャーナル |
Biochem J
|
| Abstract |
Here we show that secretory phospholipase A2 (sPLA2) that is immunochemically indistinguishable from type II sPLA2 is (i) stored in neuroendocrine cells, (ii) released in response to neurotransmitters or depolarization, and (iii) involved in the regulation of catecholamine secretion by these cells. Rat brain synaptic vesicle fractions contained PLA2 activity, which was neutralized completely by an antibody raised against rat type II sPLA2. sPLA2 immunoreactive with anti-(type II sPLA2) antibody was released from synaptosomes in response to depolarization evoked by a high concentration of potassium in the presence of Ca2+. Rat pheochromocytoma PC12 cells, which differentiated into adherent cells similar to sympathetic neurons in response to nerve growth factor, were used for the detailed analysis of the dynamics and function of sPLA2 in neuronal cells. Antibody against rat type II sPLA2 precipitated approximately 80% of the PLA2 activity in PC12 cell lysates. Transcript for type II sPLA2 was detected in PC12 cells by reverse transcriptase-PCR. When neuronally differentiated PC12 cells were stimulated with carbamylcholine or potassium, sPLA2 was released into the medium and reached a maximal approximately 40% release by 15 min. Inhibitors specific to type II sPLA2 suppressed catecholamine secretion by PC12 cells which had been activated by carbamylcholine. Furthermore, treatment of PC12 cells with exogenous type II sPLA2 alone elicited catecholamine secretion. These observations indicate that sPLA2 released from neuronal cells may regulate the degranulation process leading to release of neurotransmitters and are compatible with our earlier finding that this enzyme is involved in the degranulation of rat mast cells.
|
| 巻・号 |
318 ( Pt 2)(Pt 2)
|
| ページ |
701-9
|
| 公開日 |
1996-9-1
|
| DOI |
10.1042/bj3180701
|
| PMID |
8809065
|
| PMC |
PMC1217675
|
| MeSH |
Adrenal Gland Neoplasms
Animals
Antibodies
Brain / enzymology*
Calcium / pharmacology
Cell Differentiation
Cell Fractionation
Chromatography, Affinity
DNA Primers
Evoked Potentials / drug effects
Homeostasis
Immunoblotting
Immunohistochemistry
Kinetics
Male
Nerve Growth Factors / pharmacology
Neurons / cytology
Neurons / physiology*
Norepinephrine / metabolism*
PC12 Cells
Pheochromocytoma
Phospholipases A / biosynthesis
Phospholipases A / isolation & purification
Phospholipases A / metabolism*
Phospholipases A2
Polymerase Chain Reaction
Rats
Rats, Wistar
Synaptic Vesicles / enzymology*
Synaptosomes / enzymology*
Transcription, Genetic
|
| IF |
4.097
|
| 引用数 |
91
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
