| RRC ID |
38954
|
| Author |
Ohsawa K, Watanabe Y, Takakura A, Itoh T, Sato H.
|
| Title |
Replication of rat coronaviruses in intestinal cell line, RCN-9, derived from F344 rats.
|
| Journal |
Exp Anim
|
| Abstract |
To examine the susceptibility of the epithelial cell line to rat coronavirus (RCV), we inoculated sialodacryoadenitis virus and Parker's RCV into five cell lines; JTC-19, rat L2, LLC, RCN-9 and LBC cells originating in the lungs, intestines and mammary tumors of rodents. Both RCVs were replicated in LBC and RCN-9 cells, but not in the others. The infectivity titers of both RCVs grown in RCN-9 cells were significantly higher than those in LBC cells in every passage (2.5-3.9 log rate). Both RCVs replicated in LBC cells showed higher tropism to RCN-9 cells than to LBC cells, suggesting that RCN-9 cells are more suitable for the replication of RCVs than LBC cells. The RCN-9 cell line would be useful for the investigation of RCV infection in rodents.
|
| Volume |
45(4)
|
| Pages |
389-93
|
| Published |
1996-10-1
|
| DOI |
10.1538/expanim.45.389
|
| PMID |
8902504
|
| MeSH |
Animals
Cell Line
Coronavirus, Rat / growth & development*
Coronavirus, Rat / isolation & purification
Coronavirus, Rat / pathogenicity
Epithelial Cells
Giant Cells
Intestines / cytology
Mice
Rats
Rats, Inbred F344
Rodent Diseases / virology
Tumor Cells, Cultured
Virus Replication / physiology*
|
| IF |
1.574
|
| Times Cited |
1
|
|
WOS Category
|
VETERINARY SCIENCES
ZOOLOGY
|
| Resource |
| Human and Animal Cells |
RCN-9(RCB0511)
RCN-9(RCB0074) |
