RRC ID 38973
Author Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K.
Title Expression of neural cell adhesion molecule L1 in human lung cancer cell lines.
Journal Cell Struct Funct
Abstract To investigate neuron-specific antigens, hybridoma cells were produced between mouse spleen cells immunized with human neuroblastoma cells (IMR-32) and mouse myeloma cells. 247 hybridoma clones were harvested and one of them was further cultured for recloning. Eventually, one hybridoma clone was obtained and its antibody was designated N-A8. The characteristics of this antibody were determined by immunostaining and flow cytometry. First, the antibody recognized the surface antigens of IMR-32 cells. Second, unexpectedly, N-A8 was reactive not only with human neuroblastoma cell lines but also with human lung cancer cell lines. As analyzed by immunoprecipitation method and SDS-PAGE, the molecular size of the antigen recognized by N-A8 was 210 kDa. The antigen was then purified by affinity chromatography and identified as neural adhesion molecule L1 by amino acid sequence analysis. By the present investigation, it was clearly demonstrated that L1 is expressed in human lung cancer cells.
Volume 22(5)
Pages 511-6
Published 1997-10-1
DOI 10.1247/csf.22.511
PMID 9431455
MeSH Animals Antibodies, Monoclonal* Cell Adhesion Molecules, Neuronal / biosynthesis* Cell Adhesion Molecules, Neuronal / immunology Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique, Direct Humans Hybridomas Leukocyte L1 Antigen Complex Lung Neoplasms / genetics Lung Neoplasms / metabolism* Membrane Glycoproteins / biosynthesis* Membrane Glycoproteins / immunology Mice Mice, Inbred BALB C Multiple Myeloma / metabolism Neuroblastoma / metabolism Precipitin Tests Sequence Alignment Sequence Analysis Spleen / cytology Tumor Cells, Cultured
IF 2.429
Times Cited 19
Human and Animal Cells LA-N-2(RCB0484) Lu-139(RCB0469) NB16(RCB0478) PC-14(RCB0446) SK-N-SH(RCB0426)