| RRC ID |
38973
|
| Author |
Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K.
|
| Title |
Expression of neural cell adhesion molecule L1 in human lung cancer cell lines.
|
| Journal |
Cell Struct Funct
|
| Abstract |
To investigate neuron-specific antigens, hybridoma cells were produced between mouse spleen cells immunized with human neuroblastoma cells (IMR-32) and mouse myeloma cells. 247 hybridoma clones were harvested and one of them was further cultured for recloning. Eventually, one hybridoma clone was obtained and its antibody was designated N-A8. The characteristics of this antibody were determined by immunostaining and flow cytometry. First, the antibody recognized the surface antigens of IMR-32 cells. Second, unexpectedly, N-A8 was reactive not only with human neuroblastoma cell lines but also with human lung cancer cell lines. As analyzed by immunoprecipitation method and SDS-PAGE, the molecular size of the antigen recognized by N-A8 was 210 kDa. The antigen was then purified by affinity chromatography and identified as neural adhesion molecule L1 by amino acid sequence analysis. By the present investigation, it was clearly demonstrated that L1 is expressed in human lung cancer cells.
|
| Volume |
22(5)
|
| Pages |
511-6
|
| Published |
1997-10-1
|
| DOI |
10.1247/csf.22.511
|
| PMID |
9431455
|
| MeSH |
Animals
Antibodies, Monoclonal*
Cell Adhesion Molecules, Neuronal / biosynthesis*
Cell Adhesion Molecules, Neuronal / immunology
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique, Direct
Humans
Hybridomas
Leukocyte L1 Antigen Complex
Lung Neoplasms / genetics
Lung Neoplasms / metabolism*
Membrane Glycoproteins / biosynthesis*
Membrane Glycoproteins / immunology
Mice
Mice, Inbred BALB C
Multiple Myeloma / metabolism
Neuroblastoma / metabolism
Precipitin Tests
Sequence Alignment
Sequence Analysis
Spleen / cytology
Tumor Cells, Cultured
|
| IF |
2.429
|
| Times Cited |
19
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
LA-N-2(RCB0484)
Lu-139(RCB0469)
NB16(RCB0478)
PC-14(RCB0446)
SK-N-SH(RCB0426) |
