Reference - Detail
| RRC ID | 38976 |
|---|---|
| Author | Li TC, Yamakawa Y, Suzuki K, Tatsumi M, Razak MA, Uchida T, Takeda N, Miyamura T. |
| Title | Expression and self-assembly of empty virus-like particles of hepatitis E virus. |
| Journal | J Virol |
| Abstract |
Hepatitis E virus (HEV) is a pathogenic agent that causes fecally-orally transmitted acute hepatitis. The genome, a single-stranded positive-sense RNA, encodes three forward open reading frames (ORFs), in which an approximately 2-kb structural protein is located in the 3' end. To produce HEV-like particles the structural protein, with its N terminus truncated (amino acid residues 112 to 660 of ORF2), was expressed in insect Tn5 cells by a recombinant baculovirus. In addition to the primary translation product with a molecular mass of 58 kDa, a large amount of a further-processed molecule with a molecular mass of 50 kDa was generated and efficiently released into the culture medium. Electron microscopic observation of the culture medium revealed that the 50-kDa protein self-assembled to form empty virus-like particles (VLPs). The buoyant density of the VLPs in CsCl was 1.285 g/cm3 and their diameter was 23.7 nm, a little smaller than the 27 nm of native HEV particles secreted into the bile or stools of experimentally infected monkeys. The yield of the VLPs was 1 mg per 10(7) cells as a purified form. The particles possess antigenicity similar to that of authentic HEV particles and, consequently, they appear to be a good antigen for the sensitive detection of HEV-specific immunoglobulin G (IgG) and IgM antibodies. Furthermore, the VLP may be the most promising candidate yet for an HEV vaccine, owing to its potent immunogenicity. |
| Volume | 71(10) |
| Pages | 7207-13 |
| Published | 1997-10-1 |
| DOI | 10.1128/JVI.71.10.7207-7213.1997 |
| PMID | 9311793 |
| PMC | PMC192060 |
| MeSH | Animals Baculoviridae Bile / virology Blotting, Western Capsid / biosynthesis Cell Line Enzyme-Linked Immunosorbent Assay Feces / virology Genome, Viral Hepatitis Antibodies / blood Hepatitis E / immunology Hepatitis E / transmission Hepatitis E virus / genetics Hepatitis E virus / isolation & purification Hepatitis E virus / physiology* Humans Macaca mulatta Open Reading Frames Recombinant Proteins / biosynthesis Spodoptera Transfection Viral Structural Proteins / biosynthesis* Viral Structural Proteins / ultrastructure |
| IF | 4.501 |
| Times Cited | 221 |
| WOS Category | VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 109 |
| Altmetric score changes over past 6months | 6.0 |
| Resource | |
| Human and Animal Cells | Sf9(RCB0563) |