RRC ID 38983
Author Shoji I, Aizaki H, Tani H, Ishii K, Chiba T, Saito I, Miyamura T, Matsuura Y.
Title Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors.
Journal J Gen Virol
Abstract A baculovirus (Autographa californica nucleopolyhedrovirus) vector containing a strong promoter, the CAG promoter, was developed to introduce foreign genes into mammalian cells. Recombinant baculoviruses carrying a reporter gene under the control of the CAG promoter were inoculated into various mammalian cell lines. High-level expression was observed not only in hepatocytes but also in other non-hepatic cell lines tested. Expression of the reporter gene was detected even 14 days after infection. The infectious titre of the recovered baculoviruses decreased significantly after infection, indicating that the baculoviruses did not replicate in mammalian cells. We then compared the efficiencies of gene expression by the baculovirus vector with that of a replication-defective adenovirus vector by using the same expression unit. The same level of expression was observed in HepG2, HeLa and COS7 cells by both vectors. Efficient expression and proper processing were observed in mammalian cells infected with baculoviruses carrying genes coding for structural regions of hepatitis C virus. These results suggest that the baculovirus vector is a good tool for gene delivery into various mammalian cells in order to study the function of foreign genes.
Volume 78 ( Pt 10)
Pages 2657-64
Published 1997-10
DOI 10.1099/0022-1317-78-10-2657
PMID 9349488
MeSH Adenoviridae / genetics* Animals COS Cells Gene Expression Regulation, Viral Gene Transfer Techniques* Genes, Viral Genetic Vectors* Hepacivirus / genetics Nucleopolyhedroviruses / genetics* Spodoptera Viral Structural Proteins / genetics Virus Replication
IF 2.809
Times Cited 202
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY VIROLOGY
Resource
Human and Animal Cells