RRC ID |
38983
|
著者 |
Shoji I, Aizaki H, Tani H, Ishii K, Chiba T, Saito I, Miyamura T, Matsuura Y.
|
タイトル |
Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors.
|
ジャーナル |
J Gen Virol
|
Abstract |
A baculovirus (Autographa californica nucleopolyhedrovirus) vector containing a strong promoter, the CAG promoter, was developed to introduce foreign genes into mammalian cells. Recombinant baculoviruses carrying a reporter gene under the control of the CAG promoter were inoculated into various mammalian cell lines. High-level expression was observed not only in hepatocytes but also in other non-hepatic cell lines tested. Expression of the reporter gene was detected even 14 days after infection. The infectious titre of the recovered baculoviruses decreased significantly after infection, indicating that the baculoviruses did not replicate in mammalian cells. We then compared the efficiencies of gene expression by the baculovirus vector with that of a replication-defective adenovirus vector by using the same expression unit. The same level of expression was observed in HepG2, HeLa and COS7 cells by both vectors. Efficient expression and proper processing were observed in mammalian cells infected with baculoviruses carrying genes coding for structural regions of hepatitis C virus. These results suggest that the baculovirus vector is a good tool for gene delivery into various mammalian cells in order to study the function of foreign genes.
|
巻・号 |
78 ( Pt 10)
|
ページ |
2657-64
|
公開日 |
1997-10-1
|
DOI |
10.1099/0022-1317-78-10-2657
|
PMID |
9349488
|
MeSH |
Adenoviridae / genetics*
Animals
COS Cells
Gene Expression Regulation, Viral
Gene Transfer Techniques*
Genes, Viral
Genetic Vectors*
Hepacivirus / genetics
Nucleopolyhedroviruses / genetics*
Spodoptera
Viral Structural Proteins / genetics
Virus Replication
|
IF |
3.376
|
引用数 |
202
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
VIROLOGY
|
リソース情報 |
ヒト・動物細胞 |
RGM1(RCB0876) |