RRC ID 38984
著者 Sudo T, Kuramoto T, Komiya S, Inoue A, Itoh K.
タイトル Expression of MAGE genes in osteosarcoma.
ジャーナル J Orthop Res
Abstract Expression of MAGE genes that encode tumor-rejection antigens recognized by cytotoxic T lymphocytes with major histocompatibility complex class-I antigens was investigated in human osteosarcomas (20 cell lines and eight fresh tumor tissues). MAGE-1, 2, 3, 4, and 6 genes were expressed at the mRNA level in 11 (52.4%), 10 (47.6%), 10 (47.6%) one (4.8%), and 10 (47.6%) of 21 tumor cell lines, respectively, and in five (62.5%), six (75%), five (62.5%), one (12.5%), and five (62.5%) of eight fresh tumor tissues as determined by the reverse transcription-polymerase chain reaction method. MAGE-1 or 4 protein was detected by immunoblot analysis in eight of 11 or one of one tumor cell lines, respectively, where it was expressed at the mRNA level. Major histocompatibility complex class-I antigens were expressed in 19 of 21 tumor cell lines. These results suggest that MAGE tumor-rejection antigens are expressed in substantial numbers of osteosarcomas in a major histocompatibility class-I-restricted manner.
巻・号 15(1)
ページ 128-32
公開日 1997-1-1
DOI 10.1002/jor.1100150119
PMID 9066537
MeSH Antigens, Neoplasm / analysis Antigens, Neoplasm / genetics* Antigens, Surface / analysis Antigens, Surface / genetics Humans Immunoblotting Melanoma-Specific Antigens Multigene Family Neoplasm Proteins / analysis Neoplasm Proteins / genetics* Osteosarcoma / genetics* Osteosarcoma / metabolism RNA, Messenger / metabolism Tumor Cells, Cultured / chemistry Tumor Cells, Cultured / physiology
IF 2.728
引用数 44
WOS 分野 ORTHOPEDICS
リソース情報
ヒト・動物細胞 OST(RCB0454)