Reference - Detail
| RRC ID | 38991 |
|---|---|
| Author | Kinto N, Iwamoto M, Enomoto-Iwamoto M, Noji S, Ohuchi H, Yoshioka H, Kataoka H, Wada Y, Yuhao G, Takahashi HE, Yoshiki S, Yamaguchi A. |
| Title | Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation. |
| Journal | FEBS Lett |
| Abstract |
We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation. |
| Volume | 404(2-3) |
| Pages | 319-23 |
| Published | 1997-3-10 |
| DOI | 10.1016/s0014-5793(97)00014-8 |
| PII | S0014-5793(97)00014-8 |
| PMID | 9119087 |
| MeSH | 3T3 Cells Animals Bone Development* Cell Differentiation Cell Line Chick Embryo Coculture Techniques Embryonic Induction Fibroblasts / cytology Fibroblasts / metabolism Fibroblasts / transplantation Hedgehog Proteins Kinetics Mice Mice, Inbred C3H Mice, Nude Osteoblasts / cytology* Osteoblasts / transplantation Peptide Fragments / biosynthesis Protein Biosynthesis* Recombinant Proteins / biosynthesis Time Factors Trans-Activators* Transfection |
| IF | 3.057 |
| Times Cited | 107 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | 10T1/2(RCB0247) |