RRC ID |
39007
|
著者 |
Takahashi K, Hara E, Ogawa K, Kimura D, Fujita H, Shibahara S.
|
タイトル |
Possible implications of the induction of human heme oxygenase-1 by nitric oxide donors.
|
ジャーナル |
J Biochem
|
Abstract |
To explore the involvement of nitric oxide (NO) in the induction of heme oxygenase-1, an essential enzyme in heme catabolism, we studied the effects of NO donors on the expression of heme oxygenase-1 mRNA in HeLa human cervical cancer cells. Treatment with each of three NO donors, sodium nitroprusside, 3-morpholinosydnonimine, and S-nitroso-L-glutathione, caused noticeable increases in the expression levels of heme oxygenase- mRNA, but not heme oxygenase-2 mRNA. On the other hand, nitrite or 8-bromo cGMP exerted no noticeable effect on the levels of heme oxygenase-1 mRNA. We showed that sodium nitroprusside also increased the levels of heme oxygenase-1 protein. The sodium nitroprusside-mediated increase in heme oxygenase-1 mRNA levels was abolished by treatment with actinomycin D. The expression levels of heme oxygenase-1 mRNA were also increased by NO donors in human melanoma and neuroblastoma cell lines. Thus, the observed induction of heme oxygenase-1 may represent an important response to NO or NO-related oxidative stress. The half lives of heme oxygenase-1 and heme oxygenase-2 mRNAs were estimated to be about 3.2 h and more than 5 h, respectively.
|
巻・号 |
121(6)
|
ページ |
1162-8
|
公開日 |
1997-6-1
|
DOI |
10.1093/oxfordjournals.jbchem.a021710
|
PMID |
9354392
|
MeSH |
Cell Lineage
Enzyme Induction
HeLa Cells
Heme Oxygenase (Decyclizing) / biosynthesis*
Heme Oxygenase (Decyclizing) / genetics
Heme Oxygenase-1
Humans
Isoenzymes / biosynthesis
Isoenzymes / genetics
Membrane Proteins
Nitric Oxide / physiology*
Tumor Cells, Cultured
|
IF |
2.476
|
引用数 |
44
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
HeLa(RCB0007)
SK-N-SH(RCB0426) |