| RRC ID |
39014
|
| Author |
Shoji T, Kobori M, Shinmoto H, Tanabe M, Tsushida T.
|
| Title |
Progressive effects of phloridzin on melanogenesis in B16 mouse melanoma cells.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
When we studied the effects of polyphenols from apple fruits on melanogenesis in B16 mouse melanoma cell lines, phloridzin had dose-dependent progressive effects on melanogenesis between 10 and 500 micrograms/ml without inhibiting cell growth. At a concentration of 500 micrograms/ml, phloridzin increased the melanin content in the cells to 181% of that in control cells. In contrast, phloretin, the aglycon of phloridzin, did not activate melanogenesis in the cells and was cytotoxic at a concentration of 5 micrograms/ml. Phloridzin increased the activity of tyrosinase to 223% of that in control cells. Furthermore, phloridzin inhibited the activity of protein kinase C (PKC), which is recognized to regulate tyrosinase activity. The inhibition of PKC activity continued for 120 min from the addition of phloridzin. Therefore, we estimated that the activation of melanogenesis by phloridzin resulted from the increase of tyrosinase activity caused by the inhibition of PKC activity.
|
| Volume |
61(12)
|
| Pages |
1963-7
|
| Published |
1997-12-1
|
| DOI |
10.1271/bbb.61.1963
|
| PMID |
9438975
|
| MeSH |
Animals
Caffeic Acids / pharmacology
Cell Division / drug effects
Dose-Response Relationship, Drug
Melanins / biosynthesis*
Melanoma, Experimental / metabolism*
Mice
Monophenol Monooxygenase / metabolism
Phloretin / pharmacology
Phlorhizin / pharmacology*
Protein Kinase C / antagonists & inhibitors*
Tumor Cells, Cultured / drug effects
|
| IF |
1.516
|
| Times Cited |
14
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
B16 melanoma 4A5(RCB0557) |
