| RRC ID |
39030
|
| Author |
Tada A, Kato H, Takenaga K, Hasegawa S.
|
| Title |
Transforming growth factor beta1 increases the expressions of high molecular weight tropomyosin isoforms and vinculin and suppresses the transformed phenotypes in human lung carcinoma cells.
|
| Journal |
Cancer Lett
|
| Abstract |
Exposure of the human lung carcinoma cell line, A549 cells, to transforming growth factor beta1 (TGFbeta1) resulted in an alteration in the morphology from epithelial-like to fibroblastic flat cells. Immunofluorescent study revealed that microfilament organization was partially restored in the TGFbeta1-treated cells. We also investigated the effects of TGFbeta1 on the transformed phenotypes including the anchorage-independent growth and invasive ability of A549 cells and found that the phenotypes were strikingly suppressed in TGFbeta1-treated A549 cells. Accompanying these changes, the levels of the expression of high molecular weight tropomyosin (TM) isoforms, especially TM1 and TM2, were significantly increased in the TGFbeta1-treated cells. The expression level of vinculin was also increased in these cells. These results suggest that the increased tropomyosin and vinculin syntheses may be involved in the suppression of the transformed phenotypes of A549 cells.
|
| Volume |
121(1)
|
| Pages |
31-7
|
| Published |
1997-12-16
|
| DOI |
10.1016/s0304-3835(97)00319-4
|
| PII |
S0304-3835(97)00319-4
|
| PMID |
9459171
|
| MeSH |
Actins / analysis
Animals
Blotting, Western
Carcinoma / chemistry
Carcinoma / metabolism
Carcinoma / pathology
Cell Adhesion / drug effects
Cell Size / drug effects
Cell Transformation, Neoplastic / drug effects
Chlorocebus aethiops
Dose-Response Relationship, Drug
Humans
Lung Neoplasms / chemistry
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology
Microscopy, Fluorescence
Neoplasm Invasiveness
Transforming Growth Factor beta / pharmacology*
Tropomyosin / metabolism*
Tumor Cells, Cultured
Vero Cells
Vinculin / metabolism*
|
| IF |
7.36
|
| Times Cited |
12
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
A549(RCB0098)
Vero(RCB0001) |
