RRC ID 39030
Author Tada A, Kato H, Takenaga K, Hasegawa S.
Title Transforming growth factor beta1 increases the expressions of high molecular weight tropomyosin isoforms and vinculin and suppresses the transformed phenotypes in human lung carcinoma cells.
Journal Cancer Lett
Abstract Exposure of the human lung carcinoma cell line, A549 cells, to transforming growth factor beta1 (TGFbeta1) resulted in an alteration in the morphology from epithelial-like to fibroblastic flat cells. Immunofluorescent study revealed that microfilament organization was partially restored in the TGFbeta1-treated cells. We also investigated the effects of TGFbeta1 on the transformed phenotypes including the anchorage-independent growth and invasive ability of A549 cells and found that the phenotypes were strikingly suppressed in TGFbeta1-treated A549 cells. Accompanying these changes, the levels of the expression of high molecular weight tropomyosin (TM) isoforms, especially TM1 and TM2, were significantly increased in the TGFbeta1-treated cells. The expression level of vinculin was also increased in these cells. These results suggest that the increased tropomyosin and vinculin syntheses may be involved in the suppression of the transformed phenotypes of A549 cells.
Volume 121(1)
Pages 31-7
Published 1997-12-16
DOI 10.1016/s0304-3835(97)00319-4
PII S0304-3835(97)00319-4
PMID 9459171
MeSH Actins / analysis Animals Blotting, Western Carcinoma / chemistry Carcinoma / metabolism Carcinoma / pathology Cell Adhesion / drug effects Cell Size / drug effects Cell Transformation, Neoplastic / drug effects Chlorocebus aethiops Dose-Response Relationship, Drug Humans Lung Neoplasms / chemistry Lung Neoplasms / metabolism* Lung Neoplasms / pathology Microscopy, Fluorescence Neoplasm Invasiveness Transforming Growth Factor beta / pharmacology* Tropomyosin / metabolism* Tumor Cells, Cultured Vero Cells Vinculin / metabolism*
IF 7.36
Times Cited 12
Human and Animal Cells A549(RCB0098) Vero(RCB0001)