| RRC ID |
39066
|
| 著者 |
Lai CH, Hütter J, Hsu CW, Tanaka H, Varela-Aramburu S, De Cola L, Lepenies B, Seeberger PH.
|
| タイトル |
Analysis of Carbohydrate-Carbohydrate Interactions Using Sugar-Functionalized Silicon Nanoparticles for Cell Imaging.
|
| ジャーナル |
Nano Lett
|
| Abstract |
Protein-carbohydrate binding depends on multivalent ligand display that is even more important for low affinity carbohydrate-carbohydrate interactions. Detection and analysis of these low affinity multivalent binding events are technically challenging. We describe the synthesis of dual-fluorescent sugar-capped silicon nanoparticles that proved to be an attractive tool for the analysis of low affinity interactions. These ultrasmall NPs with sizes of around 4 nm can be used for NMR quantification of coupled sugars. The silicon nanoparticles are employed to measure the interaction between the cancer-associated glycosphingolipids GM3 and Gg3 and the associated kD value by surface plasmon resonance experiments. Cell binding studies, to investigate the biological relevance of these carbohydrate-carbohydrate interactions, also benefit from these fluorescent sugar-capped nanoparticles.
|
| 巻・号 |
16(1)
|
| ページ |
807-11
|
| 公開日 |
2016-1-13
|
| DOI |
10.1021/acs.nanolett.5b04984
|
| PMID |
26652315
|
| MeSH |
Carbohydrates / chemistry*
Cell Tracking / methods*
Glycosphingolipids / chemistry*
Glycosphingolipids / isolation & purification
Humans
Magnetic Resonance Spectroscopy
Nanoparticles / chemistry*
Silicon / chemistry
|
| IF |
11.238
|
| 引用数 |
30
|
|
WOS 分野
|
PHYSICS, APPLIED
MATERIALS SCIENCE, MULTIDISCIPLINARY
PHYSICS, CONDENSED MATTER
CHEMISTRY, MULTIDISCIPLINARY
CHEMISTRY, PHYSICAL
NANOSCIENCE & NANOTECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
GM95(RCB1026) |
