RRC ID 39077
Author Cao Y, Motomura K, Ohtsuru A, Matsumoto T, Yamashita S, Kosaka M.
Title Profilin gene expression and regulation in a temperature-sensitive breast cancer cell line: tsFT101.
Journal Pflugers Arch
Abstract The temperature-sensitive mutant cells (tsFT101) derived from a mouse mammary carcinoma cell line, FM3A, become multinucleated at a non-permissive temperature of 39 degrees C. To further understand the molecular mechanism of such cytokinetic disturbance, we examined the expression of profilin, the main regulator of the transition of globular actin (G-actin) to filamentous actin (F-actin). RT-PCR analysis of mouse profilin cDNA from tsFT101 showed a point mutation (177 A two head right arrow G) which was a wobble mutation causing no change in the encoded amino acid. The expression level of profilin mRNA was, however, diminished in cultured tsFT101 cells under non-permissive temperatures compared with wild-type FM3A cells in association with multinucleation. A stable transfection of profilin cDNA expression vector to tsFT101 cells prevented multinuclear cell formation when cultured at 39 degrees C. In contrast, antisense profilin cDNA expression vector did not alter multinuclear cell formation. The primary cause of the cytokinetic disturbance of tsFT101 cells may be due to the diminished level of profilin gene expression.
Volume 434(4)
Pages 341-5
Published 1997-8-1
DOI 10.1007/s004240050406
PII 10.1007/s004240050406
PMID 9211798
MeSH Animals Base Sequence Carcinoma / genetics* Carcinoma / pathology Carcinoma / physiopathology* Cell Nucleus / ultrastructure Contractile Proteins* DNA, Complementary / genetics Female Gene Expression* Gene Expression Regulation* Mammary Neoplasms, Animal / genetics* Mammary Neoplasms, Animal / pathology Mammary Neoplasms, Animal / physiopathology* Mice Microfilament Proteins / genetics* Microfilament Proteins / metabolism Molecular Sequence Data Point Mutation Polymerase Chain Reaction Profilins Temperature* Transcription, Genetic Tumor Cells, Cultured
IF 3.158
Times Cited 2
Human and Animal Cells FM3A FT-101(RCB0032)