| RRC ID |
39079
|
| 著者 |
Taketani Y, Miyamoto Ki, Tanaka K, Katai K, Chikamori M, Tatsumi S, Segawa H, Yamamoto H, Morita K, Takeda E.
|
| タイトル |
Gene structure and functional analysis of the human Na+/phosphate co-transporter.
|
| ジャーナル |
Biochem J
|
| Abstract |
Three lambda phage clones encompassing the Na+/phosphate co-transporter (NaPi-3) gene and its 5' flanking region were isolated from a human genomic DNA library. The gene comprises 13 exons and 12 introns and spans approx. 14 kb. All exon-intron junctions conform to the GT/AG rule. The major transcription-initiation site was determined by primer-extension analysis and is an adenosine residue 57 bp upstream of the 3' end of the first exon. There is a typical TATA box 28 bp upstream of the major transcription-initiation site and various cis-acting elements, including a cAMP-responsive element, AP-1, AP-2 and SP-1 sites in the 5' flanking region. This region also contains three direct-repeat-like sequences that resemble the consensus binding sequence for members of the steroid-thyroid hormone receptor superfamily, including vitamin D. Deletion analysis suggests that the region from nt-2409 to nt-1259 in the 5' flanking region may be involved in kidney-specific gene expression. Vitamin D responsiveness of the NaPi-3 promoter was also detected in COS-7 cells co-transfected with a human vitamin D receptor expression vector. The presence of the three vitamin D receptor- responsive elements in the NaPi-3 promoter may be important in mediating the enhanced expression of the gene by 1,25-dihydroxyvitamin D3.
|
| 巻・号 |
324 ( Pt 3)(Pt 3)
|
| ページ |
927-34
|
| 公開日 |
1997-6-15
|
| DOI |
10.1042/bj3240927
|
| PMID |
9210418
|
| PMC |
PMC1218510
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
COS Cells
Calcitriol / pharmacology
Carrier Proteins / genetics*
Carrier Proteins / metabolism
DNA, Complementary
HeLa Cells
Humans
Molecular Sequence Data
RNA Splicing
Sodium-Phosphate Cotransporter Proteins
Symporters*
Transcription, Genetic
Vitamin D / pharmacology
|
| IF |
4.097
|
| 引用数 |
45
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
COS-7(RCB0539) |
