論文 - 詳細
| RRC ID | 39092 |
|---|---|
| 著者 | Natsume T, Tomita S, Iemura S, Kinto N, Yamaguchi A, Ueno N. |
| タイトル | Interaction between soluble type I receptor for bone morphogenetic protein and bone morphogenetic protein-4. |
| ジャーナル | J Biol Chem |
| Abstract |
Bone morphogenetic proteins (BMPs) are multifunctional proteins that comprise the largest subfamily of the transforming growth factor-beta. These proteins bind to types I and II serine/threonine kinase receptors. Ligand-induced heteromeric dimerization of these receptors is the key event in initiation of biological responses. We report here large-scale expression and purification of extracellular domain of the type I receptor for BMP-2/4, using a silkworm expression system. This soluble form of BMP receptor (sBMPR) was in monomer form in solution and bound to BMP-4 but not to activin or transforming growth factor-beta1. Surface plasmon resonance studies showed that kinetic parameters of sBMPR for BMP-4 consisted of a relatively rapid association rate constant (ka = 3.81 +/- 0.19 x 10(4) s-1 M-1) and an extremely slow dissociation rate constant (kd = 3.69 +/- 0.26 x 10(-4) s-1). From these two kinetic parameters, affinity was determined to be similar to that of the intact membrane-associated receptor expressed on COS cells. sBMPR inhibited the alkaline phosphatase activity in BMP responsive cell lines such as mouse osteoblastic cell MC3T3-E1 and bone marrow stromal cell ST2. These data indicate that the extracellular domain of type I receptor for BMP-2 and BMP-4 is sufficient for high-affinity binding to its ligands and should prove useful in understanding the role of BMP-2/4 in vivo, because a suitable high-affinity anti-BMP antibody has yet to be developed. |
| 巻・号 | 272(17) |
| ページ | 11535-40 |
| 公開日 | 1997-4-25 |
| DOI | 10.1074/jbc.272.17.11535 |
| PII | S0021-9258(18)40671-0 |
| PMID | 9111068 |
| MeSH | Amino Acid Sequence Animals Binding, Competitive Biosensing Techniques Bombyx / genetics Bone Marrow / metabolism Bone Marrow Cells Bone Morphogenetic Protein 2 Bone Morphogenetic Protein 4 Bone Morphogenetic Protein Receptors, Type I Bone Morphogenetic Proteins / antagonists & inhibitors Bone Morphogenetic Proteins / metabolism* Mice Molecular Sequence Data Peptide Fragments / genetics Peptide Fragments / metabolism Protein Binding Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Receptors, Growth Factor / genetics Receptors, Growth Factor / metabolism* Recombinant Fusion Proteins / metabolism Solubility Stromal Cells / metabolism Transforming Growth Factor beta* |
| IF | 4.238 |
| 引用数 | 69 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |