| RRC ID |
39106
|
| 著者 |
Fujishige K, Yanaka N, Akatsuka H, Omori K.
|
| タイトル |
Localization of clearance receptor in rat lung and trachea: association with chondrogenic differentiation.
|
| ジャーナル |
Am J Physiol
|
| Abstract |
The lung is rich in atrial natriuretic peptide binding sites, and the majority of them are considered to be the natriuretic peptide clearance receptor (NPR-C). In this study, localization of NPR-C in the rat lung and trachea was investigated by immunohistochemical analysis with the specific antibody. Positive staining was observed in the epithelial cell layers of the trachea and bronchiole and the myocardium surrounding the pulmonary vein. Moreover, expression of NPR-C was seen in mesenchymal cells; it was especially strong in cells in the perichondrium and decreased in chondrocytes in the cartilage. Because mesenchymal cells in the perichondrium differentiate to chondrocytes, NPR-C expression is suggested to be associated with chondrogenic differentiation. The chondrogenic cell line ATDC5 was used to study NPR-C expression during chondrogenic differentiation in vitro. The undifferentiated ATDC5 cells expressed NPR-C at a much higher level than the differentiated ATDC5 cells, in accordance with the observation of the immunohistochemical analysis in the cartilage. These findings suggest that NPR-C expression is differentially regulated in chondrocytes and that the natriuretic peptides may play a role in regulating chondrocyte development in the lung.
|
| 巻・号 |
274(3)
|
| ページ |
L425-31
|
| 公開日 |
1998-3-1
|
| DOI |
10.1152/ajplung.1998.274.3.L425
|
| PMID |
9530179
|
| MeSH |
Amino Acid Sequence
Animals
Antibodies / metabolism
Cell Differentiation
Cell Line
Chondrocytes / cytology*
Chondrocytes / metabolism
Clone Cells / metabolism
Guanylate Cyclase / analysis*
Guanylate Cyclase / immunology
HeLa Cells
Humans
Lung / metabolism*
Male
Molecular Sequence Data
Rats
Rats, Inbred WKY
Receptors, Atrial Natriuretic Factor / analysis*
Receptors, Atrial Natriuretic Factor / immunology
Trachea / metabolism*
|
| IF |
3.485
|
|
WOS 分野
|
RESPIRATORY SYSTEM
PHYSIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
