論文 - 詳細
| RRC ID | 39110 |
|---|---|
| 著者 | Ogawa T, Tokuda M, Tomizawa K, Matsui H, Itano T, Konishi R, Nagahata S, Hatase O. |
| タイトル | Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The effects of three immunosuppressants (rapamycin, FK506 and cyclosporin A) on the proliferation and differentiation of rat osteoblasts-like osteosarcoma cell line, ROS 17/2.8 (ROS) cells were examined in vitro. All immunosuppressants showed a direct inhibition on the proliferation of ROS cells with different potencies. Growth inhibition by rapamycin was stronger than that by FK506 or cyclosporin A. Rapamycin caused a significant increase in alkaline phosphatase (ALP) activity and in the expression of osteopontin and osteocalcin mRNAs. FK506 caused a moderate increase in ALP activity and a decreased expression of osteopontin mRNA. Cyclosporin A caused a decrease in ALP activity and in the expression of type 1 alpha 1 collagen mRNA. Our study indicates that rapamycin directly acts on ROS cells and induces osteoblastic differentiation, however, the effect of FK506 and cyclosporin A is weak. Rapamycin significantly enhances the differentiation induced by 1,25(OH)2-vitaminD3. |
| 巻・号 | 249(1) |
| ページ | 226-30 |
| 公開日 | 1998-8-10 |
| DOI | 10.1006/bbrc.1998.9118 |
| PII | S0006-291X(98)99118-5 |
| PMID | 9705862 |
| MeSH | Animals Antibiotics, Antineoplastic / pharmacology* Calcitriol / pharmacology Cell Differentiation / drug effects Cyclosporine / pharmacology Immunosuppressive Agents / pharmacology* Osteoblasts / drug effects* Osteoblasts / metabolism Osteoblasts / pathology* Osteocalcin / biosynthesis Osteopontin Osteosarcoma / pathology* Polyenes / pharmacology* Rats Sialoglycoproteins / biosynthesis Sirolimus Tacrolimus / pharmacology Tumor Cells, Cultured |
| IF | 2.985 |
| 引用数 | 76 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ROS-17/2.8-5(RCB0462) |