| RRC ID |
39110
|
| 著者 |
Ogawa T, Tokuda M, Tomizawa K, Matsui H, Itano T, Konishi R, Nagahata S, Hatase O.
|
| タイトル |
Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
The effects of three immunosuppressants (rapamycin, FK506 and cyclosporin A) on the proliferation and differentiation of rat osteoblasts-like osteosarcoma cell line, ROS 17/2.8 (ROS) cells were examined in vitro. All immunosuppressants showed a direct inhibition on the proliferation of ROS cells with different potencies. Growth inhibition by rapamycin was stronger than that by FK506 or cyclosporin A. Rapamycin caused a significant increase in alkaline phosphatase (ALP) activity and in the expression of osteopontin and osteocalcin mRNAs. FK506 caused a moderate increase in ALP activity and a decreased expression of osteopontin mRNA. Cyclosporin A caused a decrease in ALP activity and in the expression of type 1 alpha 1 collagen mRNA. Our study indicates that rapamycin directly acts on ROS cells and induces osteoblastic differentiation, however, the effect of FK506 and cyclosporin A is weak. Rapamycin significantly enhances the differentiation induced by 1,25(OH)2-vitaminD3.
|
| 巻・号 |
249(1)
|
| ページ |
226-30
|
| 公開日 |
1998-8-10
|
| DOI |
10.1006/bbrc.1998.9118
|
| PII |
S0006-291X(98)99118-5
|
| PMID |
9705862
|
| MeSH |
Animals
Antibiotics, Antineoplastic / pharmacology*
Calcitriol / pharmacology
Cell Differentiation / drug effects
Cyclosporine / pharmacology
Immunosuppressive Agents / pharmacology*
Osteoblasts / drug effects*
Osteoblasts / metabolism
Osteoblasts / pathology*
Osteocalcin / biosynthesis
Osteopontin
Osteosarcoma / pathology*
Polyenes / pharmacology*
Rats
Sialoglycoproteins / biosynthesis
Sirolimus
Tacrolimus / pharmacology
Tumor Cells, Cultured
|
| IF |
2.985
|
| 引用数 |
76
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ROS-17/2.8-5(RCB0462) |
