| RRC ID |
39111
|
| Author |
Gohda E, Okauchi H, Iwao M, Yamamoto I.
|
| Title |
Induction of apoptosis by hepatocyte growth factor/scatter factor and its augmentation by phorbol esters in Meth A cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional cytokine with mitogenic, motogenic, and morphogenic activities. In addition, HGF/SF inhibits the proliferation of some tumor cell lines, but its mechanism remains poorly understood. We determined in this study whether HGF/SF induces cell death of a Meth A mouse sarcoma cell line in vitro, whose proliferation is remarkably suppressed by HGF/SF. Inhibition of Meth A cell growth by HGF/SF was dose-dependent and maximal at a concentration of 30 ng/ml. The percentage of dead cells increased to 22% upon treatment with 30 ng/ml of HGF/SF for 96 h, whereas that in untreated cultures was less than 5%. Staining of these cells nuclei with Hoechst 33342 revealed condensation of the chromatin and nuclear fragmentation. Gel electrophoresis of DNA from HGF/SF-treated cells showed a typical ladder pattern. Cells with a fractional DNA content also increased five-fold in the HGF/SF-treated cultures as analyzed by flow cytometry after propidium iodide staining. These are features typical of apoptosis. Concurrent addition of 12-O-tetradecanoylphorbol 13-acetate (TPA) with HGF/SF augmented the apoptosis induced by the growth factor, while TPA alone caused little death. This enhancement was largely blocked by addition of the specific protein kinase C inhibitor GF 109203X. These results indicate that HGF/SF induced the apoptotic cell death of the Meth A sarcoma cell line and that protein kinase C activation augmented the growth factor-induced apoptosis.
|
| Volume |
245(1)
|
| Pages |
278-83
|
| Published |
1998-4-7
|
| DOI |
10.1006/bbrc.1998.8416
|
| PII |
S0006-291X(98)98416-9
|
| PMID |
9535822
|
| MeSH |
Animals
Apoptosis / drug effects*
Benzimidazoles / metabolism
Cell Cycle / physiology
Cell Survival / drug effects
DNA Fragmentation / drug effects
DNA Replication / drug effects
Flow Cytometry
Fluorescent Dyes / metabolism
Hepatocyte Growth Factor / pharmacology*
Indoles / pharmacology
Maleimides / pharmacology
Mice
Protein Kinase C / antagonists & inhibitors
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured
|
| IF |
2.985
|
| Times Cited |
33
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
Meth-A(RCB0464) |
